I have used the following procedures but am still having strong primer dimer bands while the desired product is absent.
The cycling conditions for PCR program that i used were 5 min at 95C for activation followed by 35 cycles of 95oC for 30 s for denaturation, 55oC for 30 s for annealing, 72C for 30s for elongation and a final cycle 72C for 10min for final elongation
the primer used are
F: CAGCCATACAGGGCATCCAG
R: ACAGATGGGTGTGTGGGGAT
and expected pcr product size is 250
please i need a help about what is wrong