08 March 2018 5 3K Report

I have used the following procedures but am still having strong primer dimer bands while the desired product is absent.

The cycling conditions for PCR program that i used were 5 min at 95C for activation followed by 35 cycles of 95oC for 30 s for denaturation, 55oC for 30 s for annealing, 72C for 30s for elongation and a final cycle 72C for 10min for final elongation

the primer used are

F: CAGCCATACAGGGCATCCAG

R: ACAGATGGGTGTGTGGGGAT

and expected pcr product size is 250

please i need a help about what is wrong

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