Noha Said

22 Questions 20 Answers 0 Followers

Questions related from Noha Said

Could GSH level be more in CCL4 rats than negative control rats?

i compare between the antioxidant and oxidative stress level between negative control rats (saline) and CCL4 intoxicated rats. i found the level of GSH and TAC is more in toxic group than normal....

27 July 2024 2,220 0 View

What is the best buffer used in liver homogenization for measuring antioxidants and oxidative biomarkers?

what is the best buffer used in liver homogenization for measuring the following 1-total antioxidant capacity 2- reduced glutathione 3- nitric oxide 4- MAD 5- SOD 6- CAT I want to know the amount...

28 March 2023 5,887 1 View

HOW can i minimize the number of hits made by the designed primer to only the intended sequence?

i have designed a primer but i found it gives 200 hits over than the intended sequence (5 of them in the organism that i work with). this give poor pcr results. i want to avoid this but i don,t...

02 September 2018 3,220 3 View

What is the best PCR condition (PCR mix and cycles and temperature ) for amplification of a WNT10A?

I have used the following procedures but am still having strong primer dimer bands while the desired product is absent. The cycling conditions for PCR program that i used were 5 min at 95C for...

08 March 2018 2,939 5 View

How to calculate the cut off values from roc curves for determine sensitivity and specificity for a specific marker?

i want to knoe in details hoe to calcule the cut off value for roc curve and hoe to make this rock curve and the calculation formula for sensistivity and specificity

05 February 2018 3,626 6 View

Is it better to use real time with sybr green otr taq man for quantification of miRNA?

i want to know what is better to use real time with sybr green otr taq man for quantification of miRNA

14 November 2015 2,743 7 View

Does any one have an experience in working with miRNA from blood?

i will work on the miRNA extracted from blood. i want to know the best way to extract it bu using total rna extraction kit or mirna extraction kit. i want to know the precautions for its...

01 November 2015 5,501 3 View

What is the better (triazole or column spin ) for rna rxtarction?

i want to know also if it is better to make extarction to total rna from blood then isolate miRNA from it or it is better to extarct directly miRNA FROM BLOOD

25 October 2015 290 1 View

What is the best method for RNA extraction?

i want to know the best method for RNAextraction with the best result. i read about triazole and column based method. i don,t know what is the better? I want to know the precautions for keeping...

23 October 2015 2,002 12 View

Is the primer used for real time pcr different from that used in conventional pcr?

i want to know about real time pcr primer way of designing. i want the programe used for designing real time pcr primers. i want to know hoe to interpret the results. i want to know how to avoid...

06 September 2015 1,882 14 View

What is the mean for the number of SNP C677T (RS1801133) in NCBI?

i wnat to understand all the information about this SNP (C667T= c665t=rs1801133). i found several names for this polymorphism. i want to know the cause of choosing the number 667 or 665. i think...

05 February 2015 645 2 View

Should I have two annealing temps in tetre-primer ARMS-PCR or is just one enough?

I will using tetra-primer ARMS PCR for genotyping specific SNP in adiponectin gene. I have taken the 4 primers designed in a previous paper. but I noticed that the paper used one annealing temp...

05 February 2015 7,358 1 View

How can I calculate the expected size for amplification (pcr product) in a pcr reaction?

I made a pcr reaction. I want to expect the size of the product recation. I have no reference except the guide paper

30 December 2014 2,344 22 View

How can I find a specific SNP in a fASTA sequence for a gene?

i want to find a specific SNP in a fASTA sequence for a gene?

30 December 2014 8,393 5 View

Who have an experience in tetra-primer arms pcr?

it is my first time to use this technique (tetra-primer arms pcr). i want to know every thing about it. especially the percent of outer primer to inner primer. how can you calculate annealing temp...

02 November 2014 10,057 3 View

Can any one help me to obtain a used-thermal cycler device for free or with low price to my university ?

Actually, I need this thermal cycler device in my lab. But the faculty budget for the devices this year and the next year is finished. Some colleagues advised me to search for other universities...

02 November 2014 5,714 11 View

Will the PCR of my DNA be affected if I do not follow the correct steps for DNA rehydration, as outlined by my kit instructions?

I did a DNA extraction, but I am not sure if the rehydration step was good or not. The kit instructions told me to keep the DNA at 60°C for 90 minutes, but I only kept it there for 30 minutes.

19 October 2014 7,857 24 View

What will happen in a PCR reaction if the DNA sample was not present or its extraction was bad?

I did a DNA extraction for some blood samples with a ready extraction kit (solution kit, not spin column). Then I ran the DNA on the gel, but no bands appeared. I continued my work and did a PCR...

18 October 2014 9,550 18 View

Can anyone suggest how I can create a positive and negative control in PCR reaction?

I have a DNA sample and I don't know is it good or bad, I will do PCR for it. I want to be sure that the bands that will appear after PCR are the wanted amplicon and not a primer-dimer. I need a...

18 October 2014 3,544 11 View

What is the critical step for obtaining homogenous DNA samples before doing PCR?

I have frozen DNA samples and I want to do PCR on them. I want to know what I have to do with the DNA samples after getting them out of the freezer to ensure the DNA is completely soluble in the...

17 October 2014 8,348 13 View

What is the protocol of tetra-primer ARMS-PCR?

Please, I will do a tetra-primer ARMS-PCR experiment. I need any one who has experience in this method to advice me about the work way. I bought primers- master mix- template. I need the...

11 October 2014 9,495 4 View

How can I determine all the SNP for a specific gene?

i want to determine all the SNP IN IL-1 gene IN INTRON , EXONS, UTR

22 August 2014 3,771 8 View