20 Questions 17 Answers 0 Followers
Questions related from Noha Said
i compare between the antioxidant and oxidative stress level between negative control rats (saline) and CCL4 intoxicated rats. i found the level of GSH and TAC is more in toxic group than normal....
27 July 2024 1,841 0 View
what is the best buffer used in liver homogenization for measuring the following 1-total antioxidant capacity 2- reduced glutathione 3- nitric oxide 4- MAD 5- SOD 6- CAT I want to know the amount...
28 March 2023 5,808 1 View
i have designed a primer but i found it gives 200 hits over than the intended sequence (5 of them in the organism that i work with). this give poor pcr results. i want to avoid this but i don,t...
02 September 2018 3,157 3 View
I have used the following procedures but am still having strong primer dimer bands while the desired product is absent. The cycling conditions for PCR program that i used were 5 min at 95C for...
08 March 2018 2,895 5 View
i want to knoe in details hoe to calcule the cut off value for roc curve and hoe to make this rock curve and the calculation formula for sensistivity and specificity
05 February 2018 3,515 6 View
i want to know what is better to use real time with sybr green otr taq man for quantification of miRNA
14 November 2015 2,683 7 View
i will work on the miRNA extracted from blood. i want to know the best way to extract it bu using total rna extraction kit or mirna extraction kit. i want to know the precautions for its...
01 November 2015 5,447 3 View
i want to know also if it is better to make extarction to total rna from blood then isolate miRNA from it or it is better to extarct directly miRNA FROM BLOOD
25 October 2015 250 1 View
i want to know the best method for RNAextraction with the best result. i read about triazole and column based method. i don,t know what is the better? I want to know the precautions for keeping...
23 October 2015 1,935 12 View
i want to know about real time pcr primer way of designing. i want the programe used for designing real time pcr primers. i want to know hoe to interpret the results. i want to know how to avoid...
06 September 2015 1,844 14 View
i wnat to understand all the information about this SNP (C667T= c665t=rs1801133). i found several names for this polymorphism. i want to know the cause of choosing the number 667 or 665. i think...
05 February 2015 596 2 View
I will using tetra-primer ARMS PCR for genotyping specific SNP in adiponectin gene. I have taken the 4 primers designed in a previous paper. but I noticed that the paper used one annealing temp...
05 February 2015 7,301 1 View
I made a pcr reaction. I want to expect the size of the product recation. I have no reference except the guide paper
30 December 2014 2,301 22 View
i want to find a specific SNP in a fASTA sequence for a gene?
30 December 2014 8,350 5 View
it is my first time to use this technique (tetra-primer arms pcr). i want to know every thing about it. especially the percent of outer primer to inner primer. how can you calculate annealing temp...
02 November 2014 10,002 3 View
Actually, I need this thermal cycler device in my lab. But the faculty budget for the devices this year and the next year is finished. Some colleagues advised me to search for other universities...
02 November 2014 5,641 11 View
I did a DNA extraction, but I am not sure if the rehydration step was good or not. The kit instructions told me to keep the DNA at 60°C for 90 minutes, but I only kept it there for 30 minutes.
19 October 2014 7,800 24 View
I did a DNA extraction for some blood samples with a ready extraction kit (solution kit, not spin column). Then I ran the DNA on the gel, but no bands appeared. I continued my work and did a PCR...
18 October 2014 9,506 18 View
I have frozen DNA samples and I want to do PCR on them. I want to know what I have to do with the DNA samples after getting them out of the freezer to ensure the DNA is completely soluble in the...
17 October 2014 8,284 13 View
Please, I will do a tetra-primer ARMS-PCR experiment. I need any one who has experience in this method to advice me about the work way. I bought primers- master mix- template. I need the...
11 October 2014 9,439 4 View