How can I select the best plasmid after sequencing?

I have transformed a drought-related gene of 1.7kb into pGEMt vector (E.coli DH5 alpha strain). After sequencing the plasmids (3 replicates) 4 changed amino acids are visible at different...

06 February 2021 336 3 View

What can be the problem in the isolation of Plasmid?

I've tried transformation of a 1.7 kb gene with DH5 alpha strain of competent cells and also used PuC19 as a control. After selecting colonies and broth-culturing overnight, I isolated plasmid....

27 December 2020 3,596 5 View

MiRNA seq differential analysis?

Hello, I am writing to ask your help in regard to performing differential analysis to analyze miR RNA-seq data. I have miRNA seq data and I have analyzed in the following way- Initial QC - removed...

19 August 2020 5,105 7 View

How can I know the rank of each node in RPL in NetSim after simulation?

I am able to successfully run IOT RPL simulations in NetSim but in the results window, animator and plots I am not able to find the rank of the nodes. Please let me know how can I find this. Thanks

03 August 2020 3,630 3 View

How to calculate RECEIVER OPERATING CHARACTERISTICS(ROC) curves and area under the curve (AUC) of microRNA data?

Hello, I am having micro RNA data sets of control and patients now I am interested into analyzing micro RNA as a biomarker for a particular disease. I have already delta delta CT values for...

30 June 2020 7,858 2 View

I can't run Mendeley desktop on Microsoft Word 2019 MSO. Does anyone have an idea?

I have added plug-in but it does not work. it shows error 429

04 May 2020 7,810 2 View

How can i reduce primer dimers and get my cDNA PCR amplification done?

24 March 2020 1,525 3 View

I need to do Measurement of spectral emissivity by a FTIR spectrometer?

Please let me knw where i can complete this test in india

06 February 2019 6,227 2 View

How to apply statistical model in scenarios where dependent/response variable is not defined/labeled?

I have a scenario where many people are using a software and while utilizing they are assigned values between 0 to 1 based on their good or bad usage. e.g. good use would provide value to "usage"...

26 March 2018 300 4 View

What are the common steps involved in text analytics projects?

If i have to get the most possible generic steps of text analytics, what are the most commonly used steps for any text analysis model. Any help and your expert guidance/ suggestions are...

29 August 2017 9,968 8 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How to show arrows over the streamlines in CFD-post?

I follow this to make streamlines like the photos attached below: (software- CFD post-processing) First I plot the 2D streamline over a surface "streamline 1". them I plot a vector by the...

01 March 2021 8,668 3 View

Which one is easier and better at modeling slender masonry structures? ANSYS or ABAQUS?

I need to model masonry and reinforced concrete minarets for my MSc thesis. Which one is easier and better at modeling slender masonry structures? ANSYS or ABAQUS? (Or any other program for just...

01 March 2021 736 3 View

Can Virtual Servers (remote dekstop manager) run heavy ANSYS Fluent CFD simulation ?

I have a virtual machine. My host Pc specs are not that good thats why i went for virtual servers. so basically my host pc has 8gb of ram with nvidia quadro k2200 4Gb DDR5 GPU ,processor intel...

01 March 2021 8,415 1 View

How to increase mesh quality of a cfd model in ansys fluent?

How can one improve mesh skewness, orthogonality, aspect ratio?

28 February 2021 7,730 3 View

How can we simulate an hyperelastic-viscoelastic behavor with Ansys APDL?

I am working on modeling and simulation of biomecanical material behaviour, I have succeded on simulating skin using anisotropic hyperelastic material, on APDL and in our lab's finite element...

28 February 2021 552 3 View

Complex wave form in ANSYS?

Is there a way to input wave speed in a complex form on ANSYS? The wave speed I need to assign a material is 325(1+0.11i) however I have been unable to do this thus far. Failing that, is there...

27 February 2021 5,969 1 View

What is the best way to calculate the angles of twist for concrete columns subjected to torsional moment using finite element software?

In Finite element software like ANSYS , there is difficulty in obtaining direct results of twisting of concrete members because SOLID65 which is the unique element designed to represent concrete...

26 February 2021 3,806 2 View