What is the best mobile phase for analysis of minocycline, metronidazole and ciprofloxacin in human plasma?

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Urvesh D Patel

All 3 drugs are of different groups. You may not achieve accurate results...with single mobile phase...you can optimize the different proportion of solvents at different time period to elute all three compounds....

Azade Rafiee

Thank you so much for your answer @Dr. D Patel.

Do you have any suggestion about the appropriate solvent/s?

Is acetonitrile/ PBS PH:2.6 (60:40) appropriate for that reason?

Reza Sanaye

sorry, through my micro-operations on rabbits and hares, I have all but arrived at a very workable answer to your question. How might I share the results of 4 years' in vivo & in vitro & in silico experimentation ?? !