For example which method can extract polar and nonpolar constituents from medicinal plants easily?
It depends on the target compound in the medicinal plant material. Some compound are hydrophilic while others dissolve in organic solvent. I will suggest you extraction using a mixture of acetone & water and methanol or ethanol & water at different proportions and test out their effectiveness.
Thanks a lot for your guidance. If there is any chapter or book on this subject please help me.
Why do you specifically need a book or chapter? There are many papers describing the differences.
Papers like :
https://www.omicsonline.org/open-access/a-review-on-the-extraction-methods-use-in-medicinal-plants-principle-strength-and-limitation-2167-0412-1000196.php?aid=58448
Article Extraction, Isolation And Characterization Of Bioactive Comp...
Thanks a lot for your answer. I need to know about the effect of solvents on components extraction. I have heard that usp.bp references will help us. But as we are in sanction or if found, the price is so high, I need help for this or other references as book or chapter. But if there are papers on this subject, I am grateful for help.
Depending on your compound target, more polar should be fit with polar solvent and less polar corresponding with non-polar solvent, vice versa. Using Shoxlet apparatus method is simple and cheap for extraction, however, the temperature should be considered. Later, fragmentation step could use HPLC method even DAD/LC.
Good luck
Maybe you can try the MCW (methanol/chloroform/water) diphasic extraction which is reported many times in the literatuire. Chloroformic phase will include apolar constituants while methanol-water fraction will contain polar compounds
hi;
I think that methanol-water in a proportion 70:30 may be usefull in basis, but you need to fractionnate in last to relate each activity to the polarity of the extract.
sincerly
You could also look at dense phase extraction (using CO2 under high pressure). It's expensive but extracts a lot more different chemicals from the plant material.
It's very clean too. After depressurisation, any remaining CO2 evaporates.
Thanks dear Jacod.
I appreciate your guide. This method as you said is the best but so expensive and rare in Iran.
The conventional method of using expensive high pressure gas pumps is not common but it doesn't need to be very expensive.
Anyone who builds agricultural equipment could build a small device for you, using hydraulic rams and hydraulic pumps to get the pressure. These are quite cheap. The ram is used to compress your chamber with the material in it. A second ram and chamber is used to vent the CO2 into and then recompress back into a CO2 bottle.
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