How do people typically go about electroporating differentiated SH-SY5Y? I was thinking that you would have to electroporate them first then recover in media supplemented with retinoic acid. Would this or some other protocol be advisable?
Thanks,
Marty
Dear Martin,
In case your question is still of actuality, I can propose you an efficient alternative to electroporation.
We propose several transfection methods of whom Magnetofection (use of magnetic nanoparticles complexed to nucleic acid) to transfect SH-SY5Y. Actually, NeuroMag was demonstrated to work very well with DNA transfection :
- Davidson et al, Neuropsy, 2011, http://www.ncbi.nlm.nih.gov/pubmed/21716262
Beside this technology, we do have "classical" transfection reagents that were demonstrated to transfect these cells :
- with DNA and DreamFect Gold : Donelly et al, PNAS 2012, http://www.ncbi.nlm.nih.gov/pubmed/22173633
- with siRNA and Lullaby : Soundarajan et al, J Biol Chem. 2008, http://www.ncbi.nlm.nih.gov/pubmed/18032389
- with LNA and miRNA and DreamFect: Shao et al, Nucl acid Res, 2011, http://www.ncbi.nlm.nih.gov/pubmed/21266476
For your convinience you can find below a link to our web module to find bibliographic references mentioning transfection of SH-SY5Y.
would you need any information or would you like to test these reagents, please do not hesitate to contact me directly at: [email protected]
best regards and good luck in your experiment,
Cedric
http://www.ozbiosciences.com/module/cellfinder/default
http://www.ozbiosciences.com/content/13-magnetofection
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