I am working on the E.coli cell culture trying to study the impact of rheology on the mass transfer coefficient in a stirred tank reactor. What is the best method of determining the kLa at different agitation rates and air flow rates while the cells are growing inside the reactor. Can anybody please let me know.
Sohail,
There are many ways to measure the overall mass transfer coefficient kLa in gas-liquid systems : chemicals methods, physical methods, etc…
Some techniques allow to measure kLa without biological consumption of oxygen . Some others are based on direct measuring in bioprocesses.
Take a look at this review paper :
"Bioreactor scale-up and oxygen transfer rate in microbial processes: An overview":
http://sadrabiotech.com/catalog/scale%20up%20bioreactor1.pdf
Regards
Hi Mounir,
Thank you for the reply. I have already this much of information and the paper the link of which you have shared here, I have already that paper. I just wanted to know how can we have the better and more accurate determination of the kLa while the cells are continuously growing inside the bioreactor. That is what was my main concern here. Please let me know if you have any further information.
Thank you!
Regards,
Sohail
Metabolic activity is quite difficult to measure. So it is all about making a correct estimation of the oxygen mass balance which takes into account respiration of microorganisms :
https://ac.els-cdn.com/S1474667016328580/1-s2.0-S1474667016328580-main.pdf?_tid=c1db4bb0-0da1-11e8-802c-00000aab0f6c&acdnat=1518185083_d01d5907fccf9b1dbd0a6629811769de
Hi Mounir,
I agree with you it is difficult to predict the behavior of the cells inside the bioreactor. I use the dynamic gassing out and gassing in method to predict the kLa while growing cells in the reactor. Do you think it is correct estimation. Please let me know.
Thank you!
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