How to add gene sequencing sequence on NCBI?

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How can I know the stationary phase of growth of several bacteria?

How can I know the stationary phase of growth of several bacteria? We are aiming to collect exoproteome of several bacteria. We know that the ideal time to collect exoproteome is during the...

31 January 2021 4,528 10 View

How to interpret the following contour plot?

Hi. I am new to Countour plots. I need help with the understanding of the following contour plot. If someone can explain, please.

10 November 2020 3,710 1 View

Qiagen DNeasy vs QIAquick spin column?

Dear Fellows I wanted to confirm if I can use spin columns of QIAquick PCR purification kits for DNA extraction. We finished our DNeasy columns but still have reagents. And we have extra QIAquick...

03 November 2020 6,960 3 View

Which method is suitable for transgenic plant development: In-planta transformation or tissue culture-based transformation?

13 September 2020 7,717 11 View

Transmission electron microscope (TEM) service available in India?

I want my biological sample to be analysed using TEM as we don't have that instrument. So can anyone suggest any institution or lab that provides cheap TEM experimental service in India.

10 August 2020 3,807 3 View

How to find Slow and Fast light?

I am facing a problem to calculate the Slow and Fast light using Matlab. Can some share a simple code for Slow and Fast light. Thanks Xie Xie Danke.

13 June 2020 3,955 2 View

Which modelling technique to predict corona virus trends in future?

Hi, I am working on time-series data for the last 2 years. However, I have never used forecasting models. I want to enhance my statistical skills. As corona is all around us, I was thinking to...

29 March 2020 7,452 6 View

Can we assume linearity of association from this graph?

Linearity is pretty simple and obvious to guess. However, in some cases, the scenario gets totally different. IF you see the following attached graph, would you consider it linear association or...

15 January 2020 7,901 3 View

What are the skills that a PhD researcher should learn to secure a job after PhD in research field?

Hi, As the research field is really competitive, so what are the skills recommended for a Ph.D. student to learn while doing a Ph.D.? For example, I am doing a Ph.D. in environmental health...

13 January 2020 6,598 26 View

Does statistical significance matters in interpretation of results for time-series analysis?

In time-series ecological study, do we take positive results (but statistically non-significant) serious enough to be generalized in the real population?

06 January 2020 4,236 9 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Can I use FLEXBAR to detect and remove barcodes out of my short reads without demultiplexing ?

Hello, I have demultiplexed my short reads and I have removed the Illumina adapter left in the sequences and now I need to remove the barcodes used by the sequencing centre from my reads. I have...

22 February 2021 9,873 3 View