Hello, see attached paper may be useful for you.

Good luck.

Thanks but I can’t do laser microdissection. I have whole spinal cord tissue

Assuming you're using fresh tissues on ice in RNALater or some equivalent

1. Cut the bone into fragments no more than a few mm in size.

2. Freeze in liquid nitrogen and transfer to a BioPulverizer (https://biospec.com/product/biopulverizer). Hammer the sample to your heart's content.

2. Mix the pulverized sample with 0.5-1 mL Trizol.

3. Transfer the suspension to a biomasher (http://www.kimble-chase.com/advancedwebpage.aspx?cg=885&cd=3&SKUTYPE=202&SKUFLD=SKU&DM=1250&WEBID=6852) and grind the pulverized tissue for at least 3-5 minutes.

4. Extract with chloroform and precipitate with isopropanol as per standard Trizol method. Resuspend in 50 uL.

5. Depending on intended use: you may want to run the RNA over a standard total RNA purification column or, alternatively, precipitate with lithium chloride to clean up the sample.

Bone is not a friendly tissue. Step 5 is useful if your intended use is cDNA synthesis.

Thank you!