What is the best method for total RNA extraction from mice spinal cord ?

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Does anyone have an excel template for the analysis of single and multiple reference genes (2) by using the Pfaff method?

Hello, I need to analyze my Rea Time PCR data with the Pfaff method by using single or multiple reference genes (2). I would really appreciate if some can help me with an excel template. Thanks...

23 January 2019 7,759 3 View

Does anyone have an excel template for the analysis of single and multiple reference genes (2) by using the Pfaff method?

Hello, I need to analyze my Rea Time PCR data with the Pfaff method by using single or multiple reference genes (2). I would really appreciate if some can help me with an excel template. Thanks...

31 December 2018 1,676 3 View

What is the best method for total RNA extraction from mice spinal cord ?

Hello, I would like to extract total RNA from mice spinal cord. Does anyone know a method or kit for this?, Thank you!

31 December 2018 3,756 4 View

Can I run an ethidium bromide gel with SYBR green Real Time PCR products?

I want to see the products of my Real Time PCR (supermix prepared with SYBR Green I) by using an ethidium bromide gel. Have anyone done this?

04 May 2018 5,463 4 View

Does anyone know how to change the rotors of the Rotor-Gene 3000 Real-Time PCR machine?

I would like to change the 36 well rotor for the 72 well rotor, can anyone tell me how to do it?, this is not explained in the manual.

04 May 2018 3,544 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View