What is the best method for total RNA extraction from mice spinal cord ?

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Does anyone have an excel template for the analysis of single and multiple reference genes (2) by using the Pfaff method?

Hello, I need to analyze my Rea Time PCR data with the Pfaff method by using single or multiple reference genes (2). I would really appreciate if some can help me with an excel template. Thanks...

23 January 2019 7,818 3 View

Does anyone have an excel template for the analysis of single and multiple reference genes (2) by using the Pfaff method?

Hello, I need to analyze my Rea Time PCR data with the Pfaff method by using single or multiple reference genes (2). I would really appreciate if some can help me with an excel template. Thanks...

31 December 2018 1,734 3 View

What is the best method for total RNA extraction from mice spinal cord ?

Hello, I would like to extract total RNA from mice spinal cord. Does anyone know a method or kit for this?, Thank you!

31 December 2018 3,813 4 View

Can I run an ethidium bromide gel with SYBR green Real Time PCR products?

I want to see the products of my Real Time PCR (supermix prepared with SYBR Green I) by using an ethidium bromide gel. Have anyone done this?

04 May 2018 5,520 4 View

Does anyone know how to change the rotors of the Rotor-Gene 3000 Real-Time PCR machine?

I would like to change the 36 well rotor for the 72 well rotor, can anyone tell me how to do it?, this is not explained in the manual.

04 May 2018 3,621 3 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View