Can I run an ethidium bromide gel with SYBR green Real Time PCR products?

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What is the best method for total RNA extraction from mice spinal cord ?

Hello, I would like to extract total RNA from mice spinal cord. Does anyone know a method or kit for this?, Thank you!

24 January 2019 2,712 2 View

Does anyone have an excel template for the analysis of single and multiple reference genes (2) by using the Pfaff method?

Hello, I need to analyze my Rea Time PCR data with the Pfaff method by using single or multiple reference genes (2). I would really appreciate if some can help me with an excel template. Thanks...

23 January 2019 7,759 3 View

Does anyone have an excel template for the analysis of single and multiple reference genes (2) by using the Pfaff method?

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31 December 2018 1,676 3 View

What is the best method for total RNA extraction from mice spinal cord ?

Hello, I would like to extract total RNA from mice spinal cord. Does anyone know a method or kit for this?, Thank you!

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Does anyone know how to change the rotors of the Rotor-Gene 3000 Real-Time PCR machine?

I would like to change the 36 well rotor for the 72 well rotor, can anyone tell me how to do it?, this is not explained in the manual.

04 May 2018 3,544 3 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

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How do I increase the yield of my PCR product?

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Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

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Do I need to strip the PVDF membrane before staining it with colloidal gold?

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Research on Supply Chain Management in Indian context?

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PACYC PCR not working after several attempts. Would anyone have any suggestions?

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What SPSS analysis should I run with one IV (with two levels) and two DVs?

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01 March 2021 8,235 2 View

OpenBCI eeg--has anyone used them?

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Martin Bartas

Hello, Monica Hall,

yes, it is OK. You can combine both staining, but to my best of knowledge, SYBR Green I (similarly as SYBR Gold) absorbs also in UV, so I think no additional staining necessary.

Good luck!

Imtiaz Sajid

Yes, you can do this.

Abhijeet Singh

@Monika

Martin is right!

Monica Hall

Thank you all for your help. I did what Martin recommended and it worked good. No additional staining was necessary.