Hello,
I need to analyze my Rea Time PCR data with the Pfaff method by using single or multiple reference genes (2). I would really appreciate if some can help me with an excel template.
Thanks again
Attached please find the requested file. I hope it would be of help. All the best.
which depending on 1. the source of gene that used 2. reaction condition
Thank you Eman!
Hello, I would like to extract total RNA from mice spinal cord. Does anyone know a method or kit for this?, Thank you!
24 January 2019 2,712 2 View
Hello, I need to analyze my Rea Time PCR data with the Pfaff method by using single or multiple reference genes (2). I would really appreciate if some can help me with an excel template. Thanks...
31 December 2018 1,676 3 View
31 December 2018 3,756 4 View
I want to see the products of my Real Time PCR (supermix prepared with SYBR Green I) by using an ethidium bromide gel. Have anyone done this?
04 May 2018 5,463 4 View
I would like to change the 36 well rotor for the 72 well rotor, can anyone tell me how to do it?, this is not explained in the manual.
04 May 2018 3,544 3 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
02 March 2021 7,670 3 View
Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....
02 March 2021 285 2 View
So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...
02 March 2021 1,146 2 View
Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...
01 March 2021 210 1 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...
01 March 2021 8,683 4 View
Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?
01 March 2021 5,311 1 View
Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.
28 February 2021 5,008 4 View
I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...
27 February 2021 547 3 View