What is the best method for Harvesting T cells once they adhere to MSC's? Is it best to just do flow seperation?

15 March 2016 1 10K Report

I'm doing co-cultures for T cell (Jurkat) proliferation with CFSE stained and PMA/Ionomycin stimulated cells.

Cells are not needed for further culture. Is Tryple E sufficient? Or would cold PBS do the trick

Use Cell Dissociation buffer and put on your MSC's/ T cell co-culture for 1-2 minutes and remove by pipette immediately.

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