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Questions related from Flyn Mckinnirey
I am working on a variety of DNA and RNA structures (generally quite short 20-40bp) and I have seen some worrying results around stability. I understand the nature of the solution will affect the...
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I’m trying to separate 20bp circular dsDNA from remaining linear dsDNA post Exonuclease addition.Exonuclease treatment isn’t sufficient as there is relatively large background. I want to ensure...
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What's your experience with this conjugation method? I want to try a proximity ligation assay and I know there's kits out there that seem to be quite pricey and there are methods to simply bind...
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I'm currently trying to separate a protein from commercial cream (details below). I've been able to separate it using Benzethonium chloride in a BP aqueous cream but I am not getting great...
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I'm doing co-cultures for T cell (Jurkat) proliferation with CFSE stained and PMA/Ionomycin stimulated cells. Cells are not needed for further culture. Is Tryple E sufficient? Or would cold PBS do...
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Peripheral blood mononuclear cells will be used to track proliferation via CFSE in vitro. Stimulation via PMA to investigate mechanisms of immune dampening when co-cultured with MSC's.
17 January 2016 9,528 3 View
Can I use multiple donors for T cell assays? Will mixing cause unwanted stimulation?Peripheral blood mononuclear cells will be used to track proliferation via CFSE in vitro. Stimulation via PMA to...
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