Peripheral blood mononuclear cells will be used to track proliferation via CFSE in vitro. Stimulation via PMA to investigate mechanisms of immune dampening when co-cultured with MSC's.
The self/not-self differentiation would cause the cells to attack each other.
Do you want to mix the T-cells of different donors, or do you ant to mix PMBCs of donor A with T cells from donor B?
In general it is much more common to coculture activated dendritic cells with T cells. Moreover if you would like to investigate differentiation of T cells you should use naive CD4 positive T cells and activated monocyte derived dendritic cells. Otherwise you would always see a very high amount of proliferation of T cells.
Have a look at the linked paper. There we used an approach where T cell proliferation depends on the activation of DCs by LPS, but it also would work for other activating stimuli.
I hope i could help.
Patrick
Article Allergy-Protective Arabinogalactan Modulates Human Dendritic...
You will get substantial stimulation if you mix PBMCs from different donors (assuming you are talking about human donors in what is referred to in the old days as a mixed lymphocyte culture. You can get away with it if you are using specific mouse inbred strains.
Ray
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