Separation of circular and linear dsDNA methods?

17 August 2023 1 4K Report

I’m trying to separate 20bp circular dsDNA from remaining linear dsDNA post Exonuclease addition.Exonuclease treatment isn’t sufficient as there is relatively large background. I want to ensure the samples are clean from linear dsDNA at a relatively large scale for purity reasons . Gels are not an option as the recovery is low. Any thoughts on MWCO filtration or other size exclusion methods? Apart from conformation the sequences are identical so potential additives would have to distinguish between linear and circular. any suggestions?

Michael J. Benedik

Are 20bp ds DNA circles even possible? I thought for reasons of ds DNA bendability, you really can't get circles much smaller than 100bp? (ssDNA is a different matter).

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