Aditi Nadkarni

13 Questions 26 Answers 0 Followers

Questions related from Aditi Nadkarni

Optimum kit for whole cell protein extract?

Hello, in your experience what is the best kit available in the market for whole cell protein extraction from human cells and gives a high yield. We are looking for a kit that will yield at least...

04 April 2016 9,662 3 View

Do you have a detailed protocol that you could not describe well enough in the methods section of the article?

As a research scientist, I constantly struggled with not being able to thoroughly describe my protocols in depth in the Materials & Methods section because of word limits. Now as an editor for...

02 February 2016 9,349 3 View

Can a truncated transcript be identified or measured by RNA seq?

Is there a method to identify and measure truncated transcripts by RNA seq? How will the system distinguish between the full length and short transcripts?

08 August 2014 8,264 3 View

Which human cells grow best in serum-free or defined medium?

I am looking to conduct a proteomic study and need a cell line that can grow in serum free medium. Also is there a defined medium that is ideal for mass spec analysis such that the medium contents...

07 July 2014 1,725 7 View

What is the best culture media for human mammary epithelial cells?

I am planning to culture human mammary epithelial cells bought from Lonza. Unfortunately a lot of the media+growth supplements these companies sell (including Lonza, PromoCell, ATCC) significantly...

03 March 2014 7,955 2 View

What contaminant in my oligo can give an absorption peak at 240-245 nm?

I have a modified DNA oligo that started giving me trouble with ligations. Upon running it on nanodrop, I saw a strange peak at 240-245 nm along with one at 280 nm. I don't know what this peak...

11 November 2013 1,243 1 View

Can anyone help determine the cause of strange viscosity following ligation of linearized vector with annealed oligos?

I'm conducting a protocol where I anneal a set of oligos and then ligate this oligo cassette with a linearized vector using T4 DNA ligase enzyme and buffer overnight at 16 degrees. The overnight...

11 November 2013 6,232 0 View

Does anyone have experience with autism animal models?

I am looking for someone who could provide me with astrocytes or glial cells from an animal model of autism. I would really appreciate if you could get in touch with me by sending me a message for...

07 July 2013 8,614 1 View

High-throughput but relatively cheap method for measuring transcript length?

This is probably wishful thinking, but has anyone come across an assay that can measure transcript length without having to run huge gels or by RNA-seq? The former is tedious and not feasible for...

05 May 2013 9,202 5 View

Primer extension assay to distinguish a mutated sequence from a wild-type sequence using qPCR

I want to use a primer extension assay to distinguish between a mutated and wild-type sequence using qPCR. What polymerase would be ideal for such an assay? I need something that stops dead in...

10 October 2012 479 7 View

Plasmid replication assay for miniscule amounts of recovered plasmid

I have a plasmid that lacks an ori. But others in the field have seen that in some cells plasmids that lack an ori still undergo some levels of plasmid replication. For my work I want to ensure...

10 October 2012 4,472 6 View

Another cell population appears over time in a sample causing shift in flow cytometry read and problems in gating

I am analyzing and sorting human fibroblasts with GFP transfected into them. They are not fixed---just trypsinized, harvested, washed and suspended in PBS. Recently we have started to see an odd...

07 July 2012 6,646 18 View

What is the best gel extraction method for a high DNA concentration of an 11kb closed circular plasmid?

I want to purify a large amount (80 micrograms) of an 11kb plasmid by gel extraction. What method would work best?---most commercially available kits seem to swallow my big plasmid and are...

07 July 2012 5,186 17 View