Do you consider it is important to vindicate Spanish as language, in scientific issues and publication of scientific articles?

03 February 2021 3,756 4 View

Can anyone give me a guide regarding vcf files?

I have a VCF file with SNPs using the IGV program, when uploading the file some SNPs that are observed in a particular gene in IGV, when verifying it in GDV or Ensembl do not match

10 August 2020 699 3 View

Can 6M Urea inhibit lysozyme action? or this happens the other way around?

I mix both components together with other substances to treat my bacterial pellet and extract protein, but I don't know if Urea 6 M or lysozyme loses its effect

24 April 2020 4,098 14 View

Can someone please share a protocol for the production of recombinant proteins in E.coli BL21 DE3? or the use of plasmid pET 23B?

this for obtein a protein of plasmodium please

24 April 2020 4,302 3 View

Can I use an antibody with application for FC, to do IP or WB??

Hi there! I need to do immunoprecipitation and Western blot to obtain and detect a protein and I already have conjugated antibodies with FITC and PE for flow cytometry. I was wondering if there...

17 February 2020 223 3 View

Can someone explain about Different output of C/T vs A/G CSN1S2?

A NGS panel of truseq was performed, the SNP expected of CSN1S2, Ch 6, Position 87266177 are C/T, but we received A/G. There is an explain about that?

28 January 2020 1,118 2 View

Doubt about anti-mouse IgG-CFL 488 sc-362259 specificity?

I am using the rabbit anti-mouse IgG-CFL 488 sc-362259 from Santa Cruz and because some problems we performed a test using the secondary antibody without the primary and we realized this antibody...

15 January 2020 2,947 1 View

Human activities or cities layer for SDMs modelling??

Hi all, dear Scientific community, Does anyone know a server other than Worldclim to perform SDMs? Does anyone know layers for SMDs that have urban core variables or human activities? Thank you...

11 June 2019 1,109 2 View

Bibliography about Blanus cinereus and Macroprotodon?

I need to consulting different aspects about B. cinereus and M.brevis and impacts those species is suffereing for reference. Biblio I found is very basic. What papers do you use in terms of...

17 February 2019 6,225 3 View

Why would you use a non-parametric test such as Kruskal-Wallis test instead of a parametric test such as ANOVA?

We are conducting a research in the field of Behavioral Finance where some of the papers that we consulted have used the Kruskal-Wallis test for testing mean differences among groups. However we...

15 August 2018 1,385 4 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

Universal bacterial primers targeting a mRNA?

Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...

22 February 2021 9,969 3 View

How to improve RNA isolation?

Hi, I have been struggling with a weird issue for my RNA samples. I used the Qiagen RNA plus mini kit. For some reason no matter how careful I am with the washes, when I measure the RNA, I get...

22 February 2021 1,286 6 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View