Hello,
I want to study how much my cells (breast cancer cells) rely on fatty acid oxidation after inhibition of my protein of interest. I need to maintain my cells in a media that promote fatty acid oxydation for 72h.
Is it enough to use this formula: DMEM + 1%FBS + 2.5mM glucose + 0.5mM carnitine?
Do you have any other suggestion?
Thank you very much,
Mathieu