Why do cell sensitivity to antibiotic resistance increase after lentiviral infection?

03 November 2020 7,754 2 View

How to calculate the capacity of a battery from electrodes capacity?

I found that 1/Q = 1/Q+ + 1/Q- for specific capacity, I will explain my problem. Let's imagine that my positive electrode is 300 mAh/g my negative electrode capacity is also 300 mAh/g If i do a...

26 August 2020 568 3 View

Does cytotoxicity measure vary at same molecule concentration but different cells concentration ?

I have made a cytotoxicity assay using MTT at 20K cells/well in a 96 wells plate. If I do a MTT assay at 50K cells/well and at 10K cells/well, will i found the same effect ? I tried to find some...

07 April 2020 1,901 4 View

What are the affordances of video to research and practice in education?

I'm interested in digging deeper about the question : What are the affordances of video to research and practice in education? Please feel free to suggest a good paper on this theme. If it is...

27 November 2019 4,010 4 View

How to build an optimal computer for DFT calculation at a reasonable price?

I would like to build my computer from the beginning, part by part, in the purpose of DFT calculations and computational chemistry. I never did that and I'm kind of a noob in computer science. So...

22 April 2019 840 4 View

Why transformation of diploid strains in yeast is less efficient than haploid ?

Hello, For some time I realised the diploid w303 wt strain I was using was less efficient in the number of transformants I was getting. So I did a small transformation control with two different...

16 October 2018 7,486 2 View

What's the physical structure of space-time ?

A. Einstein in 1920 realized that his theory might not be complete, in the sense that Mach's principle was not consistently applied to the general relativity theory as space-time is not made of...

26 June 2018 3,196 51 View

Where does this information come from?

from uniprot : Nedd4 binds, in vitro, through the WW2 and WW3 domains, to neural isoforms of ENAH that contain the PPSY motif.

25 July 2017 9,694 1 View

Chi Square Unequal sample Size?

15 September 2016 6,243 7 View

Which IHC marker can we use to differentiate gastric cancer from normal gastric tissue ?

I have some parraffin blocks with cells extracted from gastric cancer patient and healthy patients. So far, I did not find a marker which was exclusively expressed in gastric cancer. Does anybody...

24 August 2016 8,716 3 View

Which one carries more value? A research paper in a Scopus Indexed conference proceedings or the same paper in a Journal without Scopus indexing?

I am looking for the suggestion among which one to pick from the above two categories for a research work.

27 February 2021 10,025 1 View

Which indexation is better Scopus or Web of Science?

Both are very repetitive indexing agencies.

26 February 2021 9,455 4 View

What could be reason for getting such type of graph for complex impedance in case of polymer blend films?

Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?

26 February 2021 3,487 3 View

Data Analysis K-S and S-W tests?

Hello, I have conducted analysis where the K-W and S-W have come out as p

25 February 2021 9,648 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Resistivity of PVD TiNx thin films vs. N2 flow?

In the PVD of TiN thin films (~100A), I've found the resistivity of the TiN films dropped from ~100 to 60um*cm with N2 flow increasing up to the metallic mode ending point; then the resistivity...

21 February 2021 1,864 2 View

HPLC baseline failure (ACN/Water)?

My baseline increases without any explanation, it is only fixed by changing the solution B, will it be the column? Reagents? Bomb? Detector? Solution A: H3PO4 0.01% IN WATER Solution B: H3PO4...

21 February 2021 5,656 3 View

Which is the best software or programming language (user friendly) for analysing data?

I have a basic knowledge on Python, R and MATLAB in analysing data. Among those, which is the best and user friendly? Which one should I learn deeply to analyse data?N:B: I want to learn data...

20 February 2021 3,950 15 View

Which transfection reagent is the best for plasmid transfection of MDA-MB-231 cell line, Lipofectamine based or other reagent suggestion?

I try to transfect MDA MB 231 cell line with a plasmid by PEI, unfortunately, the rate of transfection is low, and now I wanna find and order the best transfection reagent for this cell line and...

19 February 2021 828 6 View