Is Walne's media helpful? Could anyone suggest the composition exclusively for diatoms?
Dear Mr. Bose.
You can opt DAM for culturing Diatoms. Look for the attached link for further details.
actually DAM is particularly for culturing freshwater diatoms and i am searching for brackish water
Dear Mr Bose. DAM also support grow of Barckish water diatoms. Do read the attached paper for more details. hope it will resolve your question.
I could propose ESAW artificial sea water [Andersen, R.A., Berges, J.A., Harrison, P.J., Watanabe, M.M. 2005 Recipes for freshwater and seawater media. In: Algal culturing techniques. (Ed by Andersen, R.A.). Elsevier Academic Press. p.494]. We have been using it for 8 years. Never had misfires. Algae from different Oceans are growing well in our lab.
Best wishes, Nickolai
We use Bacillariophycean Medium (= Diat.) and it works in most cases. The recipes could be read at http://www.uni-goettingen.de/en/list-of-media-and-recipes/186449.html.
best wishes, Edina
f/2 guillard, I think is a good medium for diatom. you can use too K medium +Si which is maybe more "complete" : presence of 2 forms of nitrogen : NH4 and NO3. phosphate in glycerophosphate form
Romain
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