Romain Clement

2 Questions 3 Answers 0 Followers

Questions related from Romain Clement

Why my protein doesn't elute of Strep-tactin column after addition of desthiobiotin 2.5 mM (elution buffer) ?

I try to purify a membrane protein with a strep-tactin column. However, the protein doesn't elute after the addidition of dethiobiotin 2.5 mM but "elutes" during the regénération (with NaOH 0.5...

05 May 2017 9,190 2 View

Why is my protein washed away during purification?

I try to purify a protein (DEAE) but it stays with pigment and go out with wash (Pi 6.3 ; buffer pH 7.9): have you ever met this problem? I work with a diatom

12 December 2014 5,886 3 View