Dear Vijay,

Please read the following:

Cancer Stem Cell Products & Services

ProMab Biotechnologies, Inc. offers a wide line of cancer stem cell products and services including cancer stem cell media, cancer stem cell antibodies, and cancer stem cell markers. Cancer stem cells (CSCs) are hypothesized to reside within the tumor mass cell milieu, and retain certain characteristics of normal stem cells. Cancer stem cells are highly tumorigenic, in contrast to other cancer cells, persist in tumors as a distinct population, and may cause relapse by giving rise to new tumor growth with increased metastatic potential. Recent research indicates cancer stem cells display unique properties and resistance to chemotherapy and radiotherapy. This breakthrough could lead to the development of new tests for early cancer diagnosis, prognostic tests, and innovative therapeutic strategies.

Identification and isolation of cancer stem cells from either tumor cell line spheroids (tumorspheres) in vitro, or in vivo tumors, will provide valuable cell populations in order to study their origin and mechanisms of establishment, maintenance, and importantly the molecular mechanisms responsible for their conversion. ProMab’s various cancer stem cell antibodies, cancer stem cell media, cancer stem cell isolation kits and cancer stem cell markers will therefore provide the researcher with an arsenal for the development of cancer stem cell specific targeted therapies, as a means to increase survival rates amongst cancer patients in general, but especially for those at risk from tumors with high metastatic potential. See below for our cancer stem cell antibodies, cancer stem cell markers, cancer stem cell media, cancer stem cell isolation kits as well as other related products and services. In addition to our cancer stem cell line, we also offer custom monoclonal antibodies and protein expression services.

http://www.promab.com/services/cancer-stem-cell/

Isolation of cancer stem cells

Flexible: isolate cells anytime, whenever samples come in

Convenient: separate cells independently of flow sorting facilities

Versatile: large choice of cell surface markers for isolation

Diverse cell types within the tumor environment can play different roles in cancer and tumor progression. For reliable analysis of the different cell types it is crucial to work with pure cell populations as contaminating cells might skew the results.

Be able to assess the role of cancer stem cells in your research model using our MicroBead reagents and kits, which are optimized to ensure a high purity and yield of target cells. MACS®Technology allows you to isolate CSCs anytime, whenever the samples become available.

Scientific poster on breast CSC isolation and gene expression in rare cells

Report by Procoli et al. on CSCs in ovarian tumors

Scientific poster on CSC isolation

Overview of human CSC markers. Click on the image for a larger version. 

 http://www.miltenyibiotec.com/en/products-and-services/macs-cell-separation/cell-separation-reagents/cancer-stem-cells.aspx

Hoping this will be helpful,

Rafik

Hi, I'm one of the author of the paper that Rodolphe cited, actually before thinking about kits is rigth what Rodolphe said, depends on what kind of cancer you are trying to target, but even before when you refere to "Cancer cell lines" do you mean stable lines or primary? because this as well make a difference. Than depending of what you need to do, in my experience the methods most reliable for cancer stem cells growth and  selection is the spheroid culture, we have been used this methods with both sarcomas and squamous cell carcinomas with very good results, you find the protocol on the cited paper or here: 

Article Human primary bone sarcomas contain CD133+ cancer stem cells...

Article Fucosylation in cancer stem cells from oral squamous cell ca...