What is the best "in silico PCR" sites for bacteria that can be used?

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How can I merge two power regression curves into a single graph in SPSS 25?

I created Bland Altman Plot to assess the difference, But I want to visualize the difference between the two curves in the same graph.

02 July 2024 5,361 0 View

Can we transplanting wheat or corn as we do with rice?

Transplanting, a critical step in crop establishment, involves moving seedlings from a nursery to their final field location. While rice transplanting is a common practice, the feasibility of...

18 March 2024 1,254 4 View

Are there varieties or strains of wheat or corn capable of renewing their roots, as is the case with rice?

While rice has long been celebrated for its remarkable ability to regenerate roots, the same phenomenon in wheat and corn has remained relatively unexplored. Recent genetic research, however,...

18 March 2024 218 0 View

How to identify oxidation and reduction peak from CV graph?

I used 3 electrode potential state to scan the CV for electrochromic properties of metal phosphate. 1. Which peak belong to oxidation and reduction reaction in the system? 2.Is the peak on the...

01 March 2024 8,075 0 View

Any colleague interested in research collaboration and capable of conducting GC-MS?

I have aqueous and methanol (or ethanol or ethyl acetate) extracts from some local plants and am seeking a research collaborator. I will provide dry crude samples for phytochemical analysis and...

10 February 2024 2,761 0 View

I would like to learn about the chemical bonding in intermetallic compounds?

Focusing on intermetallic compounds, composed of two or more metals, this inquiry seeks to understand the nature of chemical bonds present within these alloys. Specifically, it aims to identify...

29 January 2024 8,126 4 View

Can anyone guide me rgarding the treatment of early embryonic stage of chick embryo?

I will apply a treatment by plant extract to fertilized chicken egg after 12 hrs of incubation. I need to calculate the doses and I want to know is this early embryonc stage can consider as cell...

14 January 2024 9,480 0 View

Exploring the "I": A Psychoanalytic Analysis of Selfhood and Identity in the Poetry of Sylvia Plath and Anne Sexton?

Please any relevant literature review or helpful materials on the above poets and their poetry. I am using these poems; for Sylvia Plath: - Lady Lazarus - Daddy - Mirror - Ariel - Tulips - The...

01 December 2023 10,004 2 View

How to investigate the quality of a coated layer of mxenes on a PTFE membrane?

In other words how I can ensure the coated layer adhere to the membrane strongly

30 October 2023 5,871 2 View

Extent of colon resection for cancer colon?

Extent of colon resection based on the site of the primary lesion

16 October 2023 2,787 1 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,137 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,667 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,731 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,032 3 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 725 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,056 3 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View