What is the best "in silico PCR" sites for bacteria that can be used?

More Abdallah Rafeek's questions See All

What is the most important parameter of a research work, the impact factor or the h index?

The impact factor is a measure of the importance of a journal based on the number of citations received in a year. ... Articles published in a journal with a high impact factor do not all receive...

24 February 2021 7,481 2 View

Open Source IoT Simulators supported by Machine Learning?

Dear Research Gaters, Let me first add context to the question. I am looking to simulate the waste management system and resources of a city. The hardware devices I need to simulate include,...

21 February 2021 2,333 4 View

Can medicinal plants really play an inherent rule in the COVID-19 tackle?

Numerous scientific reports cited plant extracts with potential antiviral activity and even anti-COVID-19? Why these findings are neglected? I remember very well the warning released by the WHO to...

17 February 2021 2,141 9 View

Is there any software or plugin that count the number of classes, number of methods, number of comments lines, LOC in any Java code?

I need to count the number of classes, number of methods, number of comments lines, LOC for any Java code as part of analysis process. So, I need a tool or plugin that gives me these data in any...

24 July 2020 9,958 4 View

Possibilities of formation of Octahedral complexes of Pt(II)?

Kindly I am asking for the possibilities of formation of transition metal complexes of Pt(II) with octahedral geometry. If it is possible, could you help me by referring to published papers that...

16 July 2020 427 6 View

How can I interpret coffecient differences between groups?

I am comparing my model between developed and less developed countries. Some predictors (e.g. CTR in the attached picture) significant in less developed countries model but insignificant in...

05 July 2020 5,675 3 View

Please, any one has a detailed protocol for mitochondrial memebrane potential (rh123 and PI) and SCSA in rams?

HI there, I am looking for detailed protocol for examining mitochondrial membrane potential using Rh123 and PI, also for Sperm Cell Structure Assay for rams for flow cytometrical analysis. Thank you

16 June 2020 8,808 5 View

How i can model the Channels fading (h) for users , this channels (h) follows Rayleigh distribution ?(Channel coefficient)?

If i have distances of users in vector d=[50 100 150 200 250 300 ] ; h=gain.path loss h=g . pl^-1(d) where's g is complex random variable with zero mean and variance (0.5) g~CN(0,0.5) and it...

01 May 2020 8,687 12 View

How to know what value for "y+" to aim for in ANSYS CFD?

Hello Everyone, Im performing a CFD study using ANSYS 18.1 on a 3D inverted Ahmed model to investigate the effect of an active underbody rear diffuser (Re > 1.4 e+6), my study was supposed to...

28 March 2020 9,180 11 View

Are there examples of non linear fractional integro- differential equations in physics or applied mathematics?

I am searching for applications of non-linear fractional integro-differential equations.

17 March 2020 3,537 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View