What are the challenges during isolation of Candida spp from vaginal swab?

I am preparing a protocol on Isolation of Candida spp from vaginal swab. At this moment, we don't have differential CHROMagar Candida medium in hand. We have blood agar, chocolate agar, general...

31 December 2019 833 11 View

What is the most reliable commercial ELISA kit for detection of HIV-1 gp120?

07 August 2016 8,514 4 View

How to interpret Mesh surface for protein structure in Pymol?

I'm not sure whether my question is appropriate or not. Let me clarify below. I have found two mutations in HIV-1 gp41 interact with each other. So, I prepared a homology structure. Now, in Pymol...

02 March 2016 8,253 3 View

Site directed mutagenesis kit: Which one is most satisfactory?

I have a gene of interest (HIV-1 envelope) inserted into a plasmid. Now I want to insert specify nucleotide substitution in some point by site directed mutagenesis. Please suggest me some good...

07 August 2014 8,346 6 View

How to determine the prevalence of a specific mutation from the HIV-1 database?

I am working on some specific point mutations in HIV-1 gene. I want to know the prevalence of such mutations in the published sequences.

31 December 2013 404 2 View

Protein-peptide docking Software?

I have a PDB file for viral envelope and an inhibitor peptide. Now I want to see the binding of that peptide with viral envelope. Which software to use? Can anyone suggest something easier?

09 October 2013 3,349 5 View

How to show amino acid change in protein 3D structure/ribbon model?

I have to present HIV-1 envelope protein 3D structure /ribbon model showing a certain amino acid change. I am new to this field and want to know about how to generate protein structure from...

08 September 2013 6,885 2 View

Free sequence assembly software for Mac.

I am looking for free sequence assembly software for Mac. Until now I have worked on Windows but now I have to work in Macbook. Please suggest some free software to me.

03 April 2013 2,410 4 View

How to efficiently amplify the entire env gene (envelop gene) of HIV-1 clade B from a plasma sample?

I want to first amplify the HIV-1 env gene from a patient's plasma samples and then I want to clone it into a plasmid vector. I am using the QIAamp viral RNA mini kit for the extraction of HIV-1...

31 December 2012 6,124 13 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View