Muntasir Alam

10 Questions 20 Answers 0 Followers

Questions related from Muntasir Alam

What are the challenges during isolation of Candida spp from vaginal swab?

I am preparing a protocol on Isolation of Candida spp from vaginal swab. At this moment, we don't have differential CHROMagar Candida medium in hand. We have blood agar, chocolate agar, general...

01 January 2020 916 11 View

What is the most reliable commercial ELISA kit for detection of HIV-1 gp120?

I want to detect gp120 shedding from cell surface and virion. Therefore, I am looking for an reliable commercial ELISA kit. Please suggest.

08 August 2016 8,574 4 View

How to interpret Mesh surface for protein structure in Pymol?

I'm not sure whether my question is appropriate or not. Let me clarify below. I have found two mutations in HIV-1 gp41 interact with each other. So, I prepared a homology structure. Now, in Pymol...

03 March 2016 8,314 3 View

What is the best housekeeping gene for 293T cells in western blotting?

I'm studying expression of HIV-1 envelope gene in 293T cells. I want a control for which shows same amount of samples was loaded in each well. Which housekeeping gene should I select for using as...

09 September 2014 739 3 View

Site directed mutagenesis kit: Which one is most satisfactory?

I have a gene of interest (HIV-1 envelope) inserted into a plasmid. Now I want to insert specify nucleotide substitution in some point by site directed mutagenesis. Please suggest me some good...

08 August 2014 8,407 6 View

How to determine the prevalence of a specific mutation from the HIV-1 database?

I am working on some specific point mutations in HIV-1 gene. I want to know the prevalence of such mutations in the published sequences.

01 January 2014 460 2 View

Protein-peptide docking Software?

I have a PDB file for viral envelope and an inhibitor peptide. Now I want to see the binding of that peptide with viral envelope. Which software to use? Can anyone suggest something easier?

10 October 2013 3,423 5 View

How to show amino acid change in protein 3D structure/ribbon model?

I have to present HIV-1 envelope protein 3D structure /ribbon model showing a certain amino acid change. I am new to this field and want to know about how to generate protein structure from...

09 September 2013 6,955 2 View

Free sequence assembly software for Mac.

I am looking for free sequence assembly software for Mac. Until now I have worked on Windows but now I have to work in Macbook. Please suggest some free software to me.

04 April 2013 2,499 4 View

How to efficiently amplify the entire env gene (envelop gene) of HIV-1 clade B from a plasma sample?

I want to first amplify the HIV-1 env gene from a patient's plasma samples and then I want to clone it into a plasmid vector. I am using the QIAamp viral RNA mini kit for the extraction of HIV-1...

01 January 2013 6,186 13 View