I used PFA2% for L. amazonensis promastigotes (strain pH8), the cells lost their morphology, interesting that something else I did using the same protocol with another strain and it worked.
Hello, Romario!
You look at following article, please:
Article No Need for Labels: The Autofluorescence of Leishmania taren...
Cell culture and fixation protocols
L. tarentolae promastigotes (10 ml) were cultured in T-flasks in supplemented BHI medium according to standard protocols as previously described [5], [9]. A thin layer of mid-log phase parasites was dropped on a microscope slide, dried and fixed for 15 min in one of the following solutions: (i) 100% acetone at −20°C, (ii) 20% (v/v) acetone, 80% (v/v) ethanol at −20°C, or (iii) 4% (w/v) paraformaldehyde (PFA) in phosphate buffered saline (PBS) at room temperature. Alternatively, live cell images were recorded after washing the cells three times in 1 ml PBS (1500 g, 15 min, room temperature). The exposure time for the detection of the green fluorescence was 500 ms for fixed and live cell images.
Article Imaging of the host/parasite interplay in cutaneous leishmaniasis
https://www.researchgate.net/publication/44629249_Imaging_of_the_hostparasite_interplay_in_cutaneous_leishmaniasis
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