Romário Boy

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Questions related from Romário Boy

Challenges in Assessing RNA Integrity in Leishmania: Is the Bioanalyser RIN Reliable?

I am evaluating RNA integrity from Leishmania using the Agilent 2100 Bioanalyser, but I frequently obtain low RIN values (~4-6) even when the RNA appears intact on an agarose gel. Since Leishmania...

11 February 2025 4,404 0 View

What is the best fixation for fluorescence microscopy of GFP tagged proteins in Leishmania?

I used PFA2% for L. amazonensis promastigotes (strain pH8), the cells lost their morphology, interesting that something else I did using the same protocol with another strain and it worked.

19 February 2021 6,703 3 View