I want to do a study on the osteogenic differentiation effect of an herbal extract on DPSCs, but I don't know how much FBS concentration in osteogenic medium should have. What is the best FBS concentration for osteogenic differentiation medium?
Hi! hMSCs can be cultured in DMEM with 10% fetal bovine serum, Pen/Strep, and other supplements like dexamethasone, ascorbic acid, and b-glycerophosphate as an osteogenic differentiation medium. You can check these papers: Article Effect of osteogenic differentiation medium on proliferation...
https://www.nature.com/articles/s41598-018-28699-x
You can also check some of the commercial osteogenic differentiation media:
https://www.sigmaaldrich.com/catalog/product/sigma/c28013?lang=en®ion=US
https://bioscience.lonza.com/lonza_bs/US/en/Primary-and-Stem-Cells/p/000000000000188630/Human-Mesenchymal-Stem-Cell-%28hMSC%29-Osteogenic-Differentiation-Medium-BulletKit%3Csup%3ETM%3C-sup%3E
Hi,
we used either 10% fetal bovine serum [https://doi.org/10.1155/2019/1304194] – [https://doi.org/10.3390/ijms21072368] or 10% human platelet lysate [https://doi.org/10.1038/s41598-019-53319-7].
Best regards.
Hi, we use Dulbecco's modified Eagle medium (DMEM) supplemented10% FBS, (10)mM βGP, (50)μg/mL ascorbic acid, and (10) nM dexamethasone.
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