What is the best culture methods to isolate Streptococcus agalactiae from the microbiome?

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Is there an additional benefit to using antibiotics in the Caco2 cell intestinal model other than to prevent contamination?

Is there a benefit to the Caco2 cell intestinal model from antibiotics? I was trained cell culture in the late 90's at HyClone Laboratories. We never used antibiotics. But is seems everyone does...

17 December 2023 1,172 1 View

How can I undo the removal of recent publications that belong to me?

Unfortunately I misunderstood the icon on the question that Research Gate was asking me about my authorship and I removed 17 publications that belong to me. Please help!!!

01 November 2023 9,285 1 View

Can alkyl diazirine fluorescent probes label BSA without light activation?

I am performing an in vitro in-gel labeling experiment by photo-activating a fluorescent alkyl diazirine probe with UV light in the presence of BSA to determine the required activation parameters...

07 September 2023 5,145 0 View

Is there a time constraint between miRNA mimic transfection, RNA isolation and analysis?

Hi, I am transfecting mammalian cells with a miRNA mimic. After the 24-48hr transfection incubation I proceed to RNA isolation. My question is must the RT-PCR be run straight after RNA isolation...

22 June 2023 9,252 3 View

How long does it take L-homopropargylglycine to get cleared out of cells upon integration in nascent proteins?

I am planning to incorporate 1 mM L-homopropargylglycine into HeLa cells in methionine-free DMEM media. I know that the incorporation of this alkyne-containing amino acid into nascent proteins...

12 June 2023 3,939 2 View

A Neuroscientific explanation for differences in positive emotions but not in negative emotions?

Hi everyone! I recently conducted a research study investigating the impact of virtual reality (VR) on learning and memory of difficult knowledge. The results I obtained from administering the...

28 May 2023 5,101 2 View

In ANSYS, can i do a modal analysis as a continuation to a non-lin analysis with SSTIF?

In ANSYS Classic (Mechanical APDL), is it possible to ... > model a frame, like say, a portal frame with beam elements > apply vertical loads > switch on stress-stiffening SSTIF > do...

03 April 2023 8,638 0 View

Isolated bacteria from marine sediment?

Hi, is there any pre-enrichment solution/method specify for marine bacteria? BPW is specific for enteric bacteria like Salmonella spp. I’m trying to isolate unknown bacteria from marine sediment...

02 December 2022 8,182 3 View

Need advice in Isolating E.coli from bivalves sample?

I isolated E.coli (oyster sample) from EC mug to EMB plates. I don’t know what happened to my other plate. The one with metallic green sheen is the positive control while the other plate is from...

06 August 2022 1,047 3 View

Could the Vitronectin dilution be killing my ipsc-RPE cells?

Hi, I started culturing some ipsc-RPE cells in Vitronectin XF coated 24-well plates. I've noticed that cells start detaching and and dying in areas of the well 2 days after plating. I only just...

20 April 2022 1,138 0 View

What is the difference between mathematical R^4 space and physical 4D unit space?

We assume that the difference is huge and that it is not possible to compare the two spaces. The R^4 mathematical space considers time as an external controller and the space itself is immobile in...

10 August 2024 6,678 14 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Is there an alternative to a multinomial regression which allows the DV to be non mutually exclusive?

I am trying to analyse data from a survey examining what variables affect teachers perceived barriers to incorporating technology into their classroom. I have 5 predictor variables however my DV...

06 August 2024 1,752 3 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

Who of all the Global Scientific community will help me Prof. Dr. Yoshida make way for TPEOM, MEC ~EMC to return the atmospheric gases to the norma ?

TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...

03 August 2024 2,484 1 View

I need the datasets of Microgrid for system identification?

Hi I am working on data driven model of the microgrid, for that, i need the reliable datasets for the identification of MG data driven Model. Thanks

02 August 2024 5,748 4 View

Which is more important: The human factor or the technological factor in combating climate change?

On a personal note, even though technology has attracted a lot of interest and funding to combat climate change, it is becoming more and more clear that taking care of the "Human Dimension" is...

02 August 2024 6,773 12 View

Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...

01 August 2024 4,606 7 View

Talking therapies for bipolar, psychology?

what is the best research evidence for psychological interventions for Bipolar?

01 August 2024 6,023 2 View