What is the best culture medium to grown primary human osteoblasts?

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Ralph Jeuken

We used: alpha-MEM (22–571-038, Thermo Fisher Scientific™, Waltham, MA), antibioticantimycotic 1% (v/v) (P4333, Sigma-Aldrich, Saint-Louis, MO) and EmbryoMax® ES Fetal Bovine Serum 10% (v/v) (EMD, Merck, Philadelphia, NJ) supplemented with 10 nM dexamethasone (D8893, Saint-Louis, M

O), 10 mM b-glycerol phosphate (50,020, Saint-Louis, MO) and 0.2 mM ascorbic acid 2-phosphate (A8960, Saint-Louis, MO).

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Mehdi Shakibaei

sir thanks for your answer, is your medium contain phenol red?