How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Can the Dunn's test be used as an appropriate post-hoc for the G-test?

Research Question: Are red grouse (Lagopus lagopus scotica) a statistically significant proportion of raptor (bird of prey) diets? I am planning on using a series of G-tests (determining whether...

23 February 2021 5,827 3 View

What low-cut filter (high Pass frequency) to be used for response spectrum (Tripartite plots) ?

What low-cut filter (high Pass frequency) to be used for the response spectrum (Tripartite plots)? I am currently working on differentiating between hard/soft site responses in Indo-Gangetic...

21 February 2021 9,273 2 View

Special issue on PLS-SEM in Mathematics. Are you interested?

21 February 2021 5,601 3 View

Where can the fault diagnosis data set of lithium battery be found?

Most of the data sets on the network only contain good information about the voltage and internal resistance of lithium batteries. The data of faulty battery is missing. Who can help me, I will be...

21 February 2021 8,659 1 View

How to define the sink temperature and film coefficient?

Hi, everyone. I am considering the heat conviction during compacting simulation by Abaqus. I was wondering that how to define the sink temperature and film coefficient? Is there any references...

21 February 2021 6,197 1 View

How to discriminate between Sentinel-2 Level-2A Product Discriminators?

According to the Sentinel-2 user guide here (https://sentinel.esa.int/web/sentinel/user-guides/sentinel-2-msi/naming-convention), the compact naming convention is arranged as...

21 February 2021 5,919 9 View

Are ZnS nanoparticles moisture sensitive?

Hello Everyone, I tried to form ZnS nanoparticles using the hot injection method and was able to form the same (confirmed by XRD). When I centrifuged, after the reaction I got a kind of white...

17 February 2021 1,709 3 View

Do you know the average number of proteins which can be found in osteoblasts cell culture using mass spectrometry?

We prepared three samples of osteoblasts in 3-D cell culture and we detected 101-120 different proteins. We are not sure if the detected number of the proteins is to low.

16 November 2020 4,102 1 View

Would someone be able to provide assistance with alizarin red s/mineralization assay with SaOS-2 cells?

I'm currently trying to induce and identify mineralization in my SaOS-2 cells. The issue I'm having is that the whole well will stain red, and of course this intensity depends on how long the...

26 January 2020 6,289 5 View

Would someone be able to provide assistance with alizarin red s/mineralization assay with SaOS-2 cells?

I'm currently trying to induce and identify mineralization in my SaOS-2 cells. The issue I'm having is that the whole well will stain red, and of course this intensity depends on how long the...

31 December 2019 6,629 4 View

What kind of cells are suitable for cytotoxicity test of nanoparticles?

Hi everyone, I'm currently working on bone-targeting polymeric nanoparticles for treatment of osteoporosis. Just wondering what kind of human osteoblast (or other bone cells) are best suitable for...

09 October 2019 5,799 1 View

What kind of cells are suitable for cytotoxicity test of nanoparticles?

Hi everyone, I'm currently working on bone-targeting polymeric nanoparticles for treatment of osteoporosis. Just wondering what kind of human osteoblast (or other bone cells) are best suitable for...

03 October 2019 9,077 2 View

Would someone be able to help with optimising a Western Blot for Collagen I from whole cell lysates of Saos-2 osteoblast cells?

I'm looking to run a Western Blot to identify expression of collagen type I from whole cell lysates of Saos-2 osteoblast cell lines. I've listed reagents at the bottom of the post. Experimental...

08 September 2019 2,301 6 View

What is the best way to trypsinize/disassociate bone marrow mesenchymal stem cells isolated from C-57 mice?

Hi all. I have isolated bone marrow mesenchymal stem cells from C-57 female black mice aged ~8-10 weeks. In doing so, I have scratched the plates with the needles I use to flush out the bone...

06 July 2019 867 5 View

Quantification of osteoblast staining?

Hi, I am planning on seeding rat MSCs onto 6 well plates with coverslips attached on the bottom. I then want to use stains such as Alizarin Red and Von Kossa to see the level of osteogenic...

02 February 2019 7,584 1 View

Quantification of osteoblast staining?

Hi, I am planning on seeding rat MSCs onto 6 well plates with coverslips attached on the bottom. I then want to use stains such as Alizarin Red and Von Kossa to see the level of osteogenic...

01 February 2019 2,921 1 View

Hello. Can anyone help me with osteoblast cell imaging&counting with an inverted microscope?

Hello there. I have been studying about coating of implant materials and the osteoblast cell attachment of these coatings produced by the PVD process. I couldn't obtain optical, confocal and...

31 December 2018 1,564 0 View