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Dear all, I am looking for FACS markers to characterize my rat bone marrow-derived macrophages (M0). They were differentiated from monocytes with M-CSF. I am considering to use CD11b/c and...
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Hi, what culture medium can I use to grow primary human osteoblasts from Lonza (NHOst cell system)? What supplements do I need? I am looking for a cheaper alternative than the osteoblasts medium...
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Hi, Can anyone tell me what is the seeding density for MC3T3-E1 cells per cm2? How many cells should be seeded in a 12-well plate? As general rule for a 12-well plate I found a seeding density...
21 September 2018 4,723 3 View
One day after the last split of my MC3T3-E1 culture (P23, L-MEM+ 10 % FBS+ Pen/Strep) these big particles were turning up in the cell culture flask. I can't remove them. Is this a contamination?
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