What is the procedure of removing an incorrect article from the list of my publications on Research Gate and replacing it with the correct one?

One of my recent articles was erroneously uploaded to Research Gate with incorrect title and as the printer's proof. I would like to delete it and replace it with the file as published by the...

17 December 2019 3,428 1 View

What is the difference between chemical and pythochemical analysis? Why must both the aqueous and alcoholic extract be made?

I'm currently studying on the pythochemical analysis of Lepisanthes alata for its antimicrobial properties. Please help me out with relevant facts and journals. Thank you in advance :)

17 March 2019 5,590 4 View

What is the best short dietary questionnaire to measure fruit and vegetable intake?

Hi, I am looking for a short (

11 October 2018 2,902 2 View

What is the optimum copy number of starting DNA template for PCR?

I understand the reasons why too much starting template can inhibit the formation of PCR amplicons, and I am aware of the guideline amounts of DNA in weight/volume measurements. But obviously the...

06 May 2017 5,717 5 View

Is it possible to perform bench work without a hood?

I'm planning to do an experiment in my lab (cell culturing) and the problem is that my lab does not a hood for for proper sterilization. Is there any possible was to perform lab work without using...

08 December 2016 5,797 2 View

Is it possible to use multiple stains on same cell line and analyze them by flowcytometry?

About flowcytometry.

22 August 2016 7,357 5 View

How can I quickly release poly silicon stress via annealing?

18 August 2016 5,936 2 View

Anyone know of a good online resource which allows automatic annotation of whole-exosome sequencing data (ie flags up unhealthy genes in data)?

Websites similar to www.promethese.com but for Illumina Hiseq NGS data.

17 February 2016 6,686 6 View

Does anyone have experience working with GP120 and SF9 cell transfection?

I am currently trying to perform a transfection of GP120 baculovirus into SF9 cells. However, my western blot keeps showing large numbers of nonspecific bands that show up even in the negative...

02 December 2015 5,332 1 View

How to functionalize, e.g. TiO2, nanoparticles to get free carboxylic acid groups into the surface for further coupling of substrates?

The functionalization can be developed during the synthesis of nanoparticles or once that they are already obtained through a second step in the preparation methodology.

11 December 2012 3,919 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

No title

02 March 2021 3,060 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View