I wonder what comercial cell line you suggest to study mitochondrial dysfunctions (except HEL293)? I wonder which one will be also the best to CRISPR Cas9 knock in mutation related to mitochondrial dysfunctions modelling mitochondrial diseases.
Dear Researchers, I cloned the IRF9 coding sequence into plasmid LV073. I used the Gibson assebly (NEBuilder) protocol. I cut the vector in MCS with the enzyme ScaI (blunt ends), amplified my...
26 November 2020 5,080 4 View
I have one lane and samples from two experiments (RNA and ChIP). Is there an option to sequence them together? I am afraid that RNA samples will "eat" all the reads. Thank you for any suggestion!
21 July 2020 1,846 4 View
I understand that the compressed air for the laser optics purge (first step of cleaning) such as mirrors or lenses should be "clean", but does anybody can explain me what really does it mean...
01 April 2019 8,021 5 View
I would like to buy graphite target for further DLC films creation by PLD technique, does anybody have some good experience with company offering it in terms of reliability and good...
14 March 2019 8,314 3 View
Do you know where can I buy DiFi cell line? There are not available in ATCC, ECACC or DSMZ.
20 July 2017 3,772 1 View
Because list of probes on the BeadChip contains some of MIR genes (i.e. MIR1-2, MIR1200, MIR1202, MIR1203 etc.) however, as I remember correctly - the mature microRNAs don't contain the polyA...
19 June 2016 2,867 3 View
What do you think about using 8-channel head array coil?
04 January 2014 8,779 2 View
.
26 November 2013 2,205 1 View
Dear fellows, I am currently working around specified subject. As part of my investigation I recruit volunteers, able to provide faecal tests results, suitable for ASD patients and control....
01 January 1970 6,630 3 View
I'm analysing the relation between the work with literary drama on the stage in the theatre and the theory of new philology (medieval texts). Do you have an example for it in the contemporary...
01 January 1970 8,329 2 View
What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...
03 March 2021 1,401 3 View
Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...
03 March 2021 6,193 1 View
The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...
03 March 2021 4,107 4 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...
01 March 2021 3,052 3 View
In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...
01 March 2021 1,960 4 View
I am working on roof joint and trying to simulate the behaviour in Abaqus. I am successful in running the model but the final graph does not follow the experimental curve in the negative direction...
01 March 2021 6,661 3 View
Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...
28 February 2021 3,442 2 View
Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...
28 February 2021 7,016 1 View
I wanted to add an extra parameter to the existing Johnson cook equation in the ABAQUS simulation. How to add those extra parameters. Ref: A modified Johnson-Cook material model with strain...
28 February 2021 4,841 3 View