I wonder what comercial cell line you suggest to study mitochondrial dysfunctions (except HEL293)? I wonder which one will be also the best to CRISPR Cas9 knock in mutation related to mitochondrial dysfunctions modelling mitochondrial diseases.
I used I-TASSER to predict the 3D structure of my protein of interest and PyMOL with APBS plugin to add a surface showing the electrostatic potential. Then, with PyTMs plugin, I added three...
25 January 2024 6,228 0 View
I am searching for antibody to rat brain tissue. I would prefer to purchase antibody for both Western blot and Immunohistochemistry. Thank you in advance.
01 January 2024 6,335 1 View
I am using PyMol with PyTMs plugin to visualize possible phosphorylation sites on a protein. I am wondering how I can use the program to calculate and show an optimized protein structure to avoid...
26 November 2023 7,663 1 View
Hello! I have used Promega RealTime-Glo Annexin V Apoptosis Assay but the results looks very weird and I would like to know if someone else came across that kind of trouble. The highest...
15 October 2023 4,813 0 View
I am doing immunofluorescence on tissue sections. After the deparaffinization I do the antigen retrieval, but I think is destroying some nucleolus structures I am trying to label. Any...
22 August 2023 4,503 2 View
I am looking for a measure/scale suitable for assessment in classrooms to measure cultural competences (cultural sensitivity and self-awareness)? I will be grateful for any advice :) Best...
02 January 2023 1,268 2 View
Dear Researchers, I cloned the IRF9 coding sequence into plasmid LV073. I used the Gibson assebly (NEBuilder) protocol. I cut the vector in MCS with the enzyme ScaI (blunt ends), amplified my...
26 November 2020 5,242 4 View
I have one lane and samples from two experiments (RNA and ChIP). Is there an option to sequence them together? I am afraid that RNA samples will "eat" all the reads. Thank you for any suggestion!
21 July 2020 2,043 4 View
Im planning to do the carburizing of my steel sample, but I want to prevent one part of it by copper plating and I was wandering if there is some indication about the copper coating created to be...
08 February 2020 4,958 0 View
I am looking for an accurate portable equipment to mesure PM in the ambient air.
08 January 2020 5,194 0 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...
10 August 2024 4,005 2 View
Usually, additive manufacturing techniques like SEBM, SLS, and SLM are used for interconnected porous lattice structure generation with sizes of >100–200 micrometers. Can the Fused Deposition...
09 August 2024 7,892 0 View
I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...
09 August 2024 5,048 2 View
I am trying to simulate vehicular loading on an orthotopic steel deck bridge section in ABAQUS software. The red arrow mark in the attached figure indicates the direction in which the vehicle will...
08 August 2024 719 0 View
Dear fellow researchers, I am currently working on a paper where I need to provide a reliable reference that defines and distinguishes between 3D mesh models and 3D city models. Although I am...
06 August 2024 9,986 2 View
I received an e-mail invitation to join the editorial board of Clinical Cardiology Updates. While I have published a few articles related to cardiovascular disease, there are lots of colleagues...
06 August 2024 8,981 8 View
I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...
05 August 2024 454 3 View
I am seeking experimental or applicable data for the liner (LLDPE) interface in FLAC3D numerical modeling of a large stockpile. Could you please recommend suitable references? The preferred data...
05 August 2024 3,665 0 View
I have a theoretical framework for preparing a interview guide to assess the unmet care needs of a sub population of chronically ill patients and their caregivers(dyadic interviews).
04 August 2024 8,798 7 View