Is there an antigen retrieval method that doesn't involve high temperatures?

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How to adjust an electrostatic potential surface in pyMol after adding phosphorylation sites?

I used I-TASSER to predict the 3D structure of my protein of interest and PyMOL with APBS plugin to add a surface showing the electrostatic potential. Then, with PyTMs plugin, I added three...

25 January 2024 6,228 0 View

Hi Researchers, could you recommend an antibody to the GIRK4 protein encoded by the Kcnj5 gene?

I am searching for antibody to rat brain tissue. I would prefer to purchase antibody for both Western blot and Immunohistochemistry. Thank you in advance.

01 January 2024 6,335 1 View

How to optimize a protein structure in PyMol after adding phospho residues?

I am using PyMol with PyTMs plugin to visualize possible phosphorylation sites on a protein. I am wondering how I can use the program to calculate and show an optimized protein structure to avoid...

26 November 2023 7,663 1 View

Does anyone had problem with Promega Annexin V, where it shows that living cells are dead and vice versa?

Hello! I have used Promega RealTime-Glo Annexin V Apoptosis Assay but the results looks very weird and I would like to know if someone else came across that kind of trouble. The highest...

15 October 2023 4,813 0 View

Does anybody know a good measure of cultural competence for children (7-9-year-old)?

I am looking for a measure/scale suitable for assessment in classrooms to measure cultural competences (cultural sensitivity and self-awareness)? I will be grateful for any advice :) Best...

02 January 2023 1,268 2 View

How do I clean endotoxin from plastics?

Hi, I'm looking to do an endotoxin-free plasmid DNA purification using QIAGEN EndoFree plasmid maxi kit. I need to use endotoxin free pipette tips and tubes for this. For the centrifugation steps...

15 February 2022 9,753 3 View

Comsol: How to add many point loads to a surface?

I have a very complex load with 10E6 point forces I want to apply to a surface. Is there a way to write a script in Comsol to (1) create points in the model geometry, and then (2) apply forces at...

30 August 2021 5,453 3 View

Additional bands on Western blot after protein overexpression? What are the additional products?

Dear Researchers, I cloned the IRF9 coding sequence into plasmid LV073. I used the Gibson assebly (NEBuilder) protocol. I cut the vector in MCS with the enzyme ScaI (blunt ends), amplified my...

26 November 2020 5,242 4 View

Has anyone pooled libraries from ChIP and RNA experiments in one lane for sequencing?

I have one lane and samples from two experiments (RNA and ChIP). Is there an option to sequence them together? I am afraid that RNA samples will "eat" all the reads. Thank you for any suggestion!

21 July 2020 2,043 4 View

What features (thickness?) does copper coating have to have to proprly prevent the carburizing?

Im planning to do the carburizing of my steel sample, but I want to prevent one part of it by copper plating and I was wandering if there is some indication about the copper coating created to be...

08 February 2020 4,958 0 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

Is it possible to improve the reforming section of the Haber-Bosch process without using air separation units?

A typical reforming section in a large-scale Haber-Bosch process (Figure 1) comprises two reactors. A mixture of methane (CH4) and steam (H2O) is fed into a primary reformer to produce syngas via...

01 August 2024 7,051 5 View

How to improve peaks separation in HPLC?

Hi everyone, I'd like to better separate two close peaks coming from a bioconversion sample, I'm currently using a C18 KromaPhase 4,6mm Ø.I. SCHARLAU, particle size (µm): 10, pore size (Å): 100,...

30 July 2024 435 3 View

What exactly is RAG-LLM doing? Isn’t it data engineering?

What exactly is Retrieval Augmented Generation for Large Language Model doing? Isn’t it data engineering?

30 July 2024 7,376 3 View

May I know the exact Quartile of the journal- Advanced Engineering Materials (Wiley) for material science category?

In some data sources it has been grouped in Q1 and some shows it is Q2.

29 July 2024 4,227 2 View

How to use Desmond in HPC ?

Our department has recently acquired an HPC (High-Performance Computing) system, and I'm thrilled to take my molecular dynamics calculations to the next level using Desmond. I used to run my...

28 July 2024 6,553 1 View

I need the housing or real estate prices data since 1950 till date in India. Can anyone guide how can I get this data ?

I didn't find any data source for continuous time series since 1950. NHB Residex and RBI give data from 2013 and 2010 onwards. Please guide. Thanks !!!

27 July 2024 6,271 5 View