Abcam's Caspase 3 Assay Kit (Colorimetric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectrophotometric detection of the chromophore p-nitroaniline (p-NA) after cleavage from the labeled substrate DEVD-pNA. The p-NA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm. Comparison of the absorbance of p-NA from an ap.optotic sample with an uninduced control allows determination of the fold increase in caspase 3 activity.
we are using Caspase-3 Assay kit (Beyotime Institute of Biotechnology, Nanjing, China) and it provide to us good results. the concept of the test is depend on production of spectrophotometric detection of the chromophore p-nitroaniline (p-NA) after cleavage from the labeled substrate DEVD-pNA.