Can anyone help me to extract peroxisomes ?

More Rachid Rouabhi's questions See All

Call for paper?

http://www.sciepub.com/journal/AJMBR/SpecialIssue?spid=252 call for paper to the special issue

02 March 2017 2,206 2 View

Publish with ajns a new free journal?

Dear Researchers,It is a big honor to invite you to submit a research paper, review, mini research and case report to a new launched International journal published by Tebessa University, Algeria,...

06 July 2016 2,990 4 View

About hijacked journals ?

Hi all, This is to ask everyone who know hijacked journals to put their website so that we could help and save scientific community from these persons.

01 February 2016 9,406 1 View

The link between the oxydative stress and the cancer if there is a confirmed link?

the link between the oxydative stress and the cancer if there is a confirmed link?

07 August 2015 2,693 0 View

In case of peroxysomes separation is there any simple procedure or protocol?

in case of peroxysomes separation is there any simple procedure or protocol?

07 August 2015 8,274 3 View

Can anyone tell me the difference between the isolation of enzymes from mitcochondria and peroxysomes?

02 March 2015 9,478 3 View

Can anyone tell me the difference between the isolation of enzymes from mitcochondria and peroxysomes?

Can anyone tell me the difference between the isolation of enzymes from mitcochondria and peroxysomes? Even in isolation

31 December 2014 9,214 3 View

About garlic antioxidant activities?

Most researchs talk about medicinal plants role in antioxidative stress can you send me some works on garlic roles.

11 December 2014 8,959 4 View

Is there any confirmed studies on effects of electromagnetic fields on nervous system?

10 November 2014 7,526 5 View

Are Protozoa enzymes and hormones similar to humans and other vertebrates ?

hello, for many times I read and investigate the protozoa life and ask is the enzymes and hormones similar to superior animals?

10 November 2014 984 7 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Goodwin G. Jinesh

http://onlinelibrary.wiley.com/doi/10.1002/0471143030.cb0305s06/abstract

Rachid Rouabhi

dear Goodwin thank you very much

Frank Roels

Px fractions still contain mitochondria, lysosomes, ER and debris (shown by electron microscopy). It depends on the cell source. How important impurities are, depends on the aim of your experiments.