How should I deal with missing data?

12 February 2021 5,306 4 View

No title

01 January 2021 9,970 2 View

How can I normalise cell count/protein concentration using SDS Coommasie blue stained gel?

Im quite new to cell culture and have had some inaccuracies while harvesting my cells and therefore counting them using a CASY counter. I reconstituted in what I thought to be 50,000 cells/ul,...

16 November 2020 9,829 1 View

Hazard value for malaria survival graph??

In an experiment untreated infected mice and infected treated mice observed. I recorded my data in terms of survived mice to 28 days in each group. How to calculate hazard value for treatment vs...

06 November 2020 5,548 1 View

No proteins of interest after Co-IP with Dynabeads?

Hello, I am trying to Co-IP my protein complex of interest, and every time i run it on the gel I get nothing but antibody bands. I conjugate my antibody to Dynabeads protein G overnight. I then...

29 October 2020 3,229 7 View

What kind of MMP should I use If I want to degrade rat tail collagen (Type I) gels?

I want to do MMP digestion on my collagen gels to see the effect of glycation on my collagen gels. I have difficulty to find the best MMP! some articles used MMP-1 some MMP-9 some MMP -2 and some...

28 July 2020 6,711 1 View

How can i test the Security for an algorithm in the field of IoT for a paper?

I need to know how to test the security of an implemented algorithm in the field of the IoT to state the test results in a paper. #IoT #Security #Blockchain #remix #Smart_Contract

01 July 2020 7,390 11 View

How to run Difference in Difference Different Time Periods?

Dear All, I have Panel Data fits difference in difference I regress the (Bilateral Investment Treaties-BIT) on (Bilateral FDI). BIT: is dummy taking 1 if BIT exists and Zero Otherwise....

29 May 2020 9,871 6 View

Thermodynamic properties of of the components of a certain solar thermophotovoltaic?

I am trying to simulate a solar TPV with a '1D Si/SiO2 PhC Emitter' and a single diode 'InGaAsSb pv cell'. now I am looking for 'thermal conductivity', 'Specific heat capacity' and 'density' of...

25 May 2020 7,818 3 View

How can I solve the following issue on Autodock vina "Parse error on line 2 in file "ligand.pdbqt": Unknown or inappropriate tag"?

I have all the files ligand + receptor (both in pdbqt)+ all vina files vina.exe ,vina_license+vina split +conf txt in a single folder. I keep getting this error when I try to run docking in...

05 April 2020 10,028 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Gas sensor response and recovery time independant of flow rate and chamber volume?

I am trying to obtain the response and recovery times for my gas sensor device. It appears the the response/recovery times are primarily dependant on the flow rate of the gas I inject into the...

26 February 2021 1,511 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

Open Source IoT Simulators supported by Machine Learning?

Dear Research Gaters, Let me first add context to the question. I am looking to simulate the waste management system and resources of a city. The hardware devices I need to simulate include,...

21 February 2021 2,333 4 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View