Would alcohol administration affect GAPDH expression at the protein level?

Loading control for Western Blot

22 October 2017 7,399 3 View

Starting cell count for Intracellular cytokine staining?

I am looking to stimulate negatively isolated CD4+T cells in vitro and the main cytokines of interest are IL-10 and TGF-b. My questions are: 1. What should be my starting count (of CD4+ T cells)...

11 October 2017 3,029 3 View

T cell activation protocol

I am looking to expand and activate isolated T cells in vitro and then measure secreted cytokines using Luminex (mainly IL10, IFN gamma, TGB beta, TNF alpha. There seems to be various protocols...

15 June 2017 1,625 8 View

Viral infection giving low protein content after lysis for western blot?

22 June 2016 9,736 4 View

Can variances/ SDs be treated as interval scaled?

Hi, I am analyzing temporal trends in parameters like the autocorrelation and the variance. I know that, albeit they are calculated for interval scaled variables, correlations are not interval...

11 April 2016 7,683 4 View

Recommendations homogenizing muscle for zymography?

We are using zymography to look at proteases in skeletal muscle samples. Unfortunately, while the positive controls are working, the samples with muscle extract are not. There are a couple of...

23 October 2014 8,138 6 View

Is anyone interested in taking part in a field study in project management?

I am a PhD candidate. I am writing a dissertation: "What are the factors of failure in the implementation of new project management technology utilizing dashboards as a tool to measure key...

07 August 2013 1,480 2 View

Hello from Atlanta... have you performed immunohistochemistry or Zika RNA probes?

01 January 1970 3,558 2 View

Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

What kind of intervention will be relevant to increase motivation among young sport trainees?

Any suggestion on what book to read is also welcome.

03 March 2021 1,646 4 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any suggestions or recommendations on a good publishing house based on the African for academic books in the field of Communication and media studies?

I am currently working on a book on Public Relations (from an African perspective) and the rationale behind the book is the fact that as instructors in this field, we borrow instances and case...

19 February 2021 2,482 2 View

Which books and review articles describe well the current state of deep reinforcement learning for the optimal control of physical processes?

I am going to treat in a lecture the application of deep reinforcement learning for the optimal control of physical processes. Therefore, I am looking for the best references. Besides the classics...

16 February 2021 1,006 6 View