Intranasal immunization with a DNA-vaccine - any advice about transfection reagents or adjuvants?

Hallo everyone! My lab is engaged in a project on creatin a candidate DNA-vaccine. As a spin-off of the main work we want to try intranasal immunization of mice with our experimental DNA-vaccines....

20 January 2021 8,221 2 View

Does R always evaluate all parts of a complex logical expression?

I am curious about some details of work of logical operators in R. For example, let we have following expression: A == a & B == b & C == c & D == d &.... and so on. If A == a gives FALSE, will...

19 December 2020 7,296 7 View

How can I perform pairwise protein alignments using BLAST, but without search?

I have a set of about 1000 homologous protein sequences with high diversity across various genera and families. What I need is to perform BLAST alignment of each possible sequence pair and obtain...

25 October 2020 1,199 5 View

How to move chromatograms in bioedit to make them visible?

Hello everyone, I've started doing molecular biology, rather recently. So, I've spent some time to obtain my first sequences at ABI platform and all of it were perfectly readable wheteher seq....

06 November 2017 8,312 6 View

What combination of chemotherapeutic agents for trimodal therapy of bladder cancer (MIBC) are more effectives? Is resonable use MVAC?

24 February 2017 1,652 1 View

How can I deal with the bleaching effect in FTIR spectroscopy?

19 September 2014 1,377 17 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View