What is the best and & simplest protocol for antagonism test for bacteria?

More Mushtaq Talib shawi AL- Rubaye's questions See All

Is there any bioinformatics software that can analyze several gene sequences for several samples simultaneously?

I want to analyze several genes for a set of bacteria simultaneously. Is it possible to do an analyze for that set of genes in a real time and drawing their phylogenetic tree, I mean that putting...

06 July 2017 7,042 4 View

What is the best method to find out a piece of DNA has transferred using bacteriophage or plasmid?

01 February 2017 371 2 View

Can I send no band 1 for sequencing?

02 March 2015 4,465 16 View

What is the best protocol for 16sr RNA gene PCR?

I have problem when I want to PCR 16sr RNA gene of my unknown strain. I have extracted its DNA and did PCR by using lyophilized master mix tube for PCR. The result was good but I had an extra band...

31 December 2014 1,220 11 View

What is the best technique for PCR products purification for samples with protein impurities?

My template DNA has high quantity of protein impurities I did PCR and get a good band after gel electrophoresis but I saw a smear in the gel I have checked the DNA purity with nanodrop from the...

31 December 2014 6,553 7 View

What is the best technique for Long-term storage of bacterial culture?

Except lyophilization what is the best why for bacterial culture storage?

31 December 2014 9,055 13 View

How can we detect the flagella by microscopic techniques?

11 December 2014 8,482 3 View

Why some time after DNA extraction there is no band in electrophoresis?

I extracted DNA from bacteria by using extraction Kit, by when I run the DNA on agarose gel no DNA band detected, but when I did the PCR reaction I got good result. And when I run it on agarose...

11 December 2014 4,004 9 View

How can I extract and purify cloned DNA polymerase?

Is there any protocol to extract and purify cloned DNA polymerase from bacteria?

11 December 2014 6,982 4 View

What is a suitable thermal program for 16s rRNA gene PCR in halophilic bacteria?

What is a suitable thermal program for 16s rRNA gene PCR in halophilic bacteria? Usually halophilic bacteria have high % of G+C content and this affects the thermal program for PCR. I would like a...

11 December 2014 9,565 2 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

Which statistical test to use for comparison of different groups in a categorization?

I am doing a study on how programmers with different levels of expertise choose class names. A description was provided to the participants together with a list of possible class names for the...

21 February 2021 3,662 4 View

Statistical Tests for Quantitative Study?

Hello Everyone, I have one population, sample size 200. and needed to find the correlation between Variable A and B. Also, which T-test should I choose for hypothesis testing Please advise...

21 February 2021 7,591 4 View

Delhousie Daniel Jambun

Hi. Can you elaborate more on what do you mean by antagonism test? Antagonism in what sense?

Mushtaq Talib shawi AL- Rubaye

hi Delhousie Daniel Jambun

i mean ntagonism test for searching antibiotics and bactericides produced by bacteria

I'm not entirely sure about the simplest one, however, the best and accurate protocol would be the checkerboard method using broth microdilution. Apart from this, the overlay agar method might be worth trying too. Patch individual bacterial isolate on each side of the agar and let them grow for a few days. Then, overlay with soft agar containing pathogen of interest. If you're looking for antagonistic activity, it would be expected that the zone of inhibition is reduced at the side where the two zones are supposed to come into contact with each other.