Delhousie Daniel Jambun

6 Questions 11 Answers 0 Followers

Questions related from Delhousie Daniel Jambun

Can bacteria mutate to become resistant to phyotchemicals that act directly on their membrane by "dissolving" them?

One example of such a phytochemical might arguably be dodecenal. Due to the nature of this compound having a lipophilic tail and hydrophilic head, the tail can sit itself in the bacteria membrane...

11 November 2014 2,921 6 View

When you screen for antibiofilm properties of phytochemicals, what aspects do you look at?

For me, I'd look for inhibition of biofilm formation as well as biofilm biomass reduction through crystal violet assay and also cell viability reduction through BacLight Live Dead staining method....

11 November 2014 1,850 5 View

What are the optimal scanning electron microscopy parameters when viewing bacteria?

I would like to evaluate the effects of several antimicrobials on bacterial cell morphology. Can anyone suggest what are the optimal settings for the scanning electron microscopy? The parameters...

09 September 2014 4,417 9 View

What are the available tests for saponins? Are all saponins biosurfactants?

I conducted water extraction on my plant and deduced that saponins are present. As far as I know, foam test and blood haemolysis test are generally used to detect saponins. Are these the only...

08 August 2013 9,388 2 View

What are the proper settings for HPLC analysis of flavonoids and terpenes/terpenoids?

I had performed a hexane extraction on my plant. Through TLC, I had identified several spots. Then, I conducted preparative TLC to extract each spot and test their antimicrobial and antioxidant...

08 August 2013 335 12 View

What is a good positive control for a biofilm assay?

I am conducting biofilm assays, more specifically biofilm inhibition and biofilm disruption. The pathogens that I'm working on are Staphylococcus aureus, Enterococcus faecalis and Pseudomonas...

03 March 2013 3,621 9 View