What is the appropriate raw CT value of GAPDH in qPCR?

05 September 2016 1 5K Report

I am getting CT value of 18-21 in 1 qPCR run and 17-18 in another. what is the desirable range?

As mentioned above, Ct value depends on the amount of cDNA in the sample. Normally we use 1 microgram of RNA to make cDNA and get the Ct value of GAPDH between 18-22.

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Science method

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