I am trying to grow Embryonic stem cells and and I have read in the literature that rho inhibitor are used to preserve "stemness" and viability. These were the most commonly cited chemicals, but I have no experience with these pathways.
Hello
Both are good and demonstrated to increase the viability of newly isolated stem cells . for that , you can choose the best for your experiment and available in your lab ,
Good Luck
http://www.animalreproductionscience.com/article/S0378-4320(15)00185-2/pdf
Hi,
I used thiazovivin in my former iPSC experiments and it worked fine. AND it is cheaper than Y-27632. But some iPSC lines grew better with Y-27632, but it depends on the specific iPSC line rather than on the Rock inhibitor itself. As you are working with ESCs, thiazivin work fine and save you some money.
Good luck
i recommend you Y27. In organoids the cells die easily and this inhibitor works very well.
does anybody observe difference in hESC colony shape with or withour ROCKi Y27?
Rock inhibitor does not preserve "stemness." It preserves survival during the passaging of colonies and allows for the survival of single cells that normally would commence apoptosis due to cell adhesion signalling being disturbed.
I have not noted any changes in colony morphology because I never use Y-27632 for longer than 12-18 hours. There is no benefit to using it any longer than that. In fact, a one hour exposure is sufficient.
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