Givon Sanati

9 Questions 6 Answers 0 Followers

Questions related from Givon Sanati

Can quiescent stem cells secrete exosomes?

I am studying exosome release of a different variety of stem cells. I know that proliferating stem cells/progenitors do secrete exosomes. However, I was unsure about quiescent stem cells.

12 May 2016 5,665 9 View

What is a cheap and easy way to separate cancer cells from tissue cell suspension?

I am separating ovarian cancer cells from primary cell tissue and I do not have a FACS machine or any magnetic bead. Is there any suggestions to separate the fibroblast and mesenchymal cells from...

09 March 2016 9,503 2 View

What is the a better rho inhibitor Y27632 or Thiazovivin?

I am trying to grow Embryonic stem cells and and I have read in the literature that rho inhibitor are used to preserve "stemness" and viability. These were the most commonly cited chemicals, but I...

26 February 2016 1,103 7 View

What is a good differentiation protocol for glioblastoma stem cells to differentiation?

I am generating u87 Gliblastoma stem cells and I want to see their differentiation ability.

26 January 2016 5,456 1 View

What is a common and simple functional analysis for testing the potency of a cancer stem cell?

I have tested my glioblastoma U87 cancer cell lines for cancer stem cell markers. I wish to see a way other than stem cell marker expression, the potency of my cells.

19 January 2016 5,324 3 View

What is a simple technique to measure cologenicity in cancer stem cells?

I have obtained some glioblastoma cancer stem cells and I want to determine their self-renewal potency based on the colony forming ability. If there is any other assays to determine potency for...

16 January 2016 546 2 View

How do you separate quiescent cells from senescent cells?

I am trying to separated stem cells from old differentiated senescent to enrich a population of my culture. Stem cells are usually in a quiescent state, so to separate would be ideal for me.

21 December 2015 8,199 4 View

What is the optimal extracellular matrix for Neural stem cell niche?

I am trying to find the optimal neural stem cell 3D niche where the cells reside in the brain. Has anyone read any paper would be the best extracellular matrix (laminen, collagen, fibronectin,...

07 August 2015 9,938 1 View

Can a single neural stem cell/ Neural Progenitor form a neurospheres on its own or must there be several in order to initiate a paracrine response ?

I any designing a microwell assay to measure spheroid forming potency of neural stem cells on a single cell basis. I could not find any papers that specifically listed whether it is or is not...

14 July 2015 8,776 7 View