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Questions related from Givon Sanati
I am studying exosome release of a different variety of stem cells. I know that proliferating stem cells/progenitors do secrete exosomes. However, I was unsure about quiescent stem cells.
12 May 2016 5,628 9 View
I am separating ovarian cancer cells from primary cell tissue and I do not have a FACS machine or any magnetic bead. Is there any suggestions to separate the fibroblast and mesenchymal cells from...
09 March 2016 9,459 2 View
I am trying to grow Embryonic stem cells and and I have read in the literature that rho inhibitor are used to preserve "stemness" and viability. These were the most commonly cited chemicals, but I...
26 February 2016 1,076 7 View
I have tested my glioblastoma U87 cancer cell lines for cancer stem cell markers. I wish to see a way other than stem cell marker expression, the potency of my cells.
19 January 2016 5,276 3 View
I have obtained some glioblastoma cancer stem cells and I want to determine their self-renewal potency based on the colony forming ability. If there is any other assays to determine potency for...
16 January 2016 505 2 View
I am trying to separated stem cells from old differentiated senescent to enrich a population of my culture. Stem cells are usually in a quiescent state, so to separate would be ideal for me.
21 December 2015 8,164 4 View
I am trying to find the optimal neural stem cell 3D niche where the cells reside in the brain. Has anyone read any paper would be the best extracellular matrix (laminen, collagen, fibronectin,...
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