I am studying exosome release of a different variety of stem cells. I know that proliferating stem cells/progenitors do secrete exosomes. However, I was unsure about quiescent stem cells.
Hello Dear Givon,
My answer is yes. For exosome isolation we generally incubate cells at 70-80% confluence in FBS free media. So we are incubating a good number of cells to secrete proteins and we don't even expect them to proliferate them during this FBS free condition, we just need them metabolically active. As stem cells can come back from quiscence, so they might be releasing some proteins/exosomes for sure that they don't die and can revert back when conditions are favorable. If you wil do proteomic profiling of these cells then you are definitely gonna find some exosomes or proteins but profile might be different from healthy cells.
Hope it helps !
Hi Ajay
Thank you for your message. Do you have any protocol or papers that you have read during the past as a reference regarding this phenomena? I would very much appreciate it.
Thanks again.
Hello Givon,
Yes, there are articles in very reputed journals like cancer research which report that senescence can induce or sometimes increase the secretion of exosomes. Please have a look on the links provided below, they will be of great help to you.
https://www.biochemistry.org/Portals/0/Conferences/abstracts/SA138/SA138P047.pdf
http://www.ncbi.nlm.nih.gov/pubmed/18829542
http://cancerres.aacrjournals.org/content/68/19/7864.full.pdf
Cheers!
Greeting
May i add that, exosome get formed as a normal biological micro-organelles, either during cell life span ( as a sequence of plasma membrane recycling), or as a container for intercellular signal transfer. So, i think it is logic to be produced even in quiescent cells
You could find a full description on exosome on : Free Encycloopedia
Good luck
Hello
I agree with all researchers .
see all link and attachments
Hope help you , Good Luck
http://austinpublishinggroup.com/clinical-pathology/fulltext/ajcp-v1-id1001.php
All stem cells secrete exosomes !!
http://www.futuremedicine.com/doi/pdf/10.2217/rme.11.35
All the best !!
I understand stem cells secrete exosomes. However, when stem cells are in the quiescent state, the are metabolically inactive and non dividing or "sleeping." During this remission, I am wondering if anyone knows that there are exosome secretion because to be logically it seems that they do not because they are dormant.
Hello Givon,
They do secrete exosomes even in this sleping condition but the profile gets altered as compared to the normal profile. Even in sleeping conditions our body remains active to maintain the necessary reservoirs and pool for survival. Same can be expected at the level of a single cell, may be stem cell in this case. I have attached papers above for the same.
Cheers !
- Badges
- Science topic
- Similar topics
- Chemistry
- Pharmacology
- Pharmaceuticals
- Drugs