I have been attempting to do some comparative studies in ASIA context, and below are some challenges faced?

01 February 2017 7,633 5 View

Recently, we have a viral video on somebody committing suicide, now we have a rape broadcasted life?

Internet or Facebook Anthropology? Can Facebook do something about these?

31 December 2016 1,955 3 View

SBQ-R Suicide Questionnaire?

Any idea what the author try to imply between the choice "Never" and "No chance at all" for SBQ-R under item 4? How likely is it that you will attempt suicide someday? (Circle only one): 0 =...

10 November 2016 9,712 6 View

Is Alkaline water good for the body?

Is there any scientific evidence endorsing that drinking alkaline water is good for health? Regards.

09 October 2016 5,386 6 View

Is there any similarity between ITS (Interpersonal Theory of Suicide, Joiner, 2010) and Durkheim's Suicide (1897)?

ITS was written from the psychology perspective, while Durkheim's Suicide Model was written more from the sociology perspective. Are there any similarities?

08 September 2016 2,688 7 View

How to measure Religiosity?

08 September 2016 6,755 6 View

Are there still websites or social networking sites promoting or facilitating suicide (directly or indirectly)?

Are there still any websites or social networking sites (e.g. Facebook, Google) that promotes or facilitates (directly or indirectly) suicide? I am trying to compile a list of such websites and...

08 September 2016 6,404 1 View

What does Durkheim mean when he used the word "chaotic" to develop his "anomic" category of suicide?

Nearly every article published on suicide from sociology viewpoint will quote Durkheim, and some will start fitting types of suicides in this 21st century lifestyle into one of his four categories...

07 August 2016 1,224 7 View

Any researchers in Malaysia public universities interested to be an online tutor on suicidality during this coming semester break?

Pls email me at [email protected]. Many thanks.

07 August 2016 4,464 0 View

Is there any good book on EXISTENTIALISM?

I am doing some research on Religiosity and Suicide. As I study deeper, it takes me to areas of Western Civilisation, Sociology, Anthropology, Philosophy, Psychology, etc. Is there any good books...

07 August 2016 493 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

What to do with absence of fluorescence label in Fucci2a mouse-derived fibroblast culture?

Dear all, I have harvested fibroblast cells from R26p-Fucci2 mouse embryos (mouse line as described in this paper: https://dev.biologists.org/content/140/1/237) and cultured them on plastic,...

17 February 2021 3,368 2 View

Abaqus: deactivate displacement boundary condition?

I am running analysis of a cross-shaped sample that is prescribed a displacement along x and y-axes. I have run several jobs with this model and had no issues. However, the current set of...

15 February 2021 1,680 8 View

How to make Fast Green FCF from powder?

Hi everyone We need Fast Green to electroporate plasmid into mice embryos, therefore we simply need it to see some color in the liquid we are electroporating so that electroporation is more...

07 February 2021 1,671 2 View

Statistical methods for comparing gene expression curves?

Hi all, I have generated two gene expression curves by measuring bioluminescence every 10 mins for 12 hrs. These curves tend to overlap at at some time points, while a difference can be seen at...

02 February 2021 2,613 7 View

Help needed: How to map AA, AG, and GG genotypes on to polymorphism information?

Dear Colleagues, I am new to genes and genotyping and I need help. I am trying to figure our how AA, AG, and GG genotypes map on to A1 for DRD2/ANKK1 Taq1A polymorphism(rs1800497) and Val or MET...

02 February 2021 3,861 3 View

Should GFP sequence be cut together with the IRES sequence?

Hello. I have a viral plasmid which contains a bicistronic region: CMVprmtr-GFP-IRES-PuroR. I would like to cut out the GFP sequence to get rid of the green fluorescence. In this case, would it be...

02 February 2021 1,644 7 View

How to do DNA normalization based on qPCR values?

Hi Everyone: I have a question regarding to qPCR. I am trying to do RT-PCR, and I want to have equal amount of input DNA for my RT-PCR, so I normalize my DNA input based on qPCR values of a...

31 January 2021 6,977 4 View

How to make a pushover Analysis of a reinforced cantilever beam in ABAQUS?

Hello everyone, Im doing a pushover Analysis of a cantilever beam in ABAQUS, made of reinforced concrete, but my model doesnt seem to converge proberly. The program simply aborts before hitting a...

15 January 2021 1,627 3 View