How about lasergene?
There are quite a few free online tools, SIFT and PolyPhen2 are probably the most widely used, also Mutationtaster is not uncommon.
I’m trying to reproduce the acousto-optic coupling simulation in the paper Frequency–angular resolving LiDAR using chip-scale acousto-optic beam steering. LNOI(lithium niobate on insulator) is...
13 August 2024 9,795 0 View
In the Itasca FLAC3D Documentation, it provides the expression of plasticity multiplier regarding Mohr-Coulomb criterion directly, without a detailed derivation process. Could anyone share the...
12 July 2024 7,850 0 View
I am conducting a study on sandstone samples and need to accurately differentiate between tuffaceous matrix and clay matrix when examining thin sections under a polarized light microscope. While...
20 June 2024 3,270 0 View
It is generally believed that time dilation and length contraction is a geometric effect, is there anyone has tried to explain the time dilation and length contraction effect in a geometric...
05 June 2024 1,591 6 View
Delay could induce the periodic behaviors through Hopf bifurcation, Are there any other bifurcations induced by delay that can lead to periodic behavior? Are there some references?
16 May 2024 10,003 1 View
The equation in fig.1 can be transformed to the one in fig.2. Using ‘atan’, we can turn the periodic function ‘tan’,which is on the left of Eq.1, to the sum of ‘atan’ and l*π. However, for the...
14 May 2024 8,585 4 View
Tansverse resonance condition for the single layer(FIg.3) waveguide has bee deduced, as shown in Fig.2, which contains the phase shifts cause by reflection and optical path difference. Only light...
24 April 2024 1,748 4 View
Thank you!
10 April 2024 8,336 1 View
In my experiment, I need to add three monomers to the solvent. Adjust the content of different monomers to obtain the materials I need. Now, I have a rough idea of what the polymer content is for...
06 April 2024 9,568 2 View
As the article Ligands for CsPbBr 3 perovskite quantum dots: The stronger the better? reported, Reaction Yield= mpurified*w%/n*MPb where mpurified is the mass of the dried quantum dots, w% is the...
01 March 2024 8,981 2 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...
28 July 2024 6,938 1 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View
I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...
25 July 2024 5,001 2 View
In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....
24 July 2024 8,076 3 View