Shirong Zheng

5 Questions 9 Answers 0 Followers

Questions related from Shirong Zheng

Could bone tissue be saved in 70% EtOH after decalcification before processing?

I got the mouse tibia and femur tissue harvested, fixed with formalin 48h and decalcified with 14% EDTA 72h before paraffin embedding. This protocol works well for both HE and IHC. Now, for the...

19 October 2020 5,570 1 View

Is this a glomerulus?

For testing the pluropotency of iPSC, we derived embryoid bodies from human iPSC to identify the three germ layers in these embryoid bodies. I have no problem to identify all three germ layers in...

23 February 2017 4,805 3 View

Could multi-proteins in PI3K/AKT pathway molecules be ubiquitinated and degradated under one treatment in cells?

In western blot, I observed the protein decrease in total AKT, PI3K 110b, PI3K classIII and some others, while actin and COX IV remains almost no change. Could this be via proteasome mechanism for...

15 March 2016 8,256 6 View

In a multimer analysis of vWF, how can I understand this western blot protocol?

Von Willebrand factor (vWF) is a large glycoprotein, composed of a seried of multumers with MW ranging from about 800 to 20,000 kDa. For analysis of this multimer with western blot, a special...

02 November 2015 7,030 4 View

What is some good software to predict protein function based on a sequenced gene mutation? Any free online?

How about lasergene?

25 April 2014 7,732 1 View