I am looking at producing lipid vesicles to use in an experiment. The paper I am following talks about mixing lipids 40 mol % and 60 mol % to total of 50 uM phospholipids.
That means 2:3 molar ratio of two lipids, in your specific example there was 20 uM of one phospholipid and 30 uM of another. Thus each vesicle contains 40 mol% of one phospholipid and 60mol% of another.
Mole percent is the percentage that the moles of a particular component are of the total moles that are in a mixture.The sum of the mole fractions for each component in a solution is equal to 1. Mole percent is equal to the mole fraction for the component multiplied by 100 % and the sum of the mole percentages for each component in a solution is equal to 100 %.
You can understand this in terms of number of molecules. Consider a mixture composed two lipids A and B. 40 mol% of A and 60 mol% B implies that out of 100 lipid molecules 40 molecules are A and 60 molecules are B. In general number of molecules NA of any lipid A of x gm can be calculated as NA = (N/MA)x , MA is the molecular weight of A and N is the Avogadro's number. Similarly for B. This way you can make mixture of two lipids of any ratio.
Tara.... Any percentage form means the amount of the specific component to the total using the same units. As an example: 10 mol% means 10 moles of that components mixed with 90 mole of the rest of components. For your case, the mixture total number of components is 50 umol. 40 mol% means 20 umol of that component with 30 umol of the rest of components. ... Good luck